Thermosensitive and mucoadhesive hydrogel containing curcumin-loaded lipid-core nanocapsules coated with chitosan for the treatment of oral squamous cell carcinoma

被引:30
|
作者
Ortega, Ana [1 ]
da Silva, Ayana Bretas [1 ]
da Costa, Leidyana Moraes [2 ]
Zatta, Kelly Cristine [1 ]
Onzi, Giovana Ravizzoni [1 ]
da Fonseca, Francisco Noe [3 ]
Guterres, Silvia Staniscuaski [1 ,2 ]
Paese, Karina [1 ]
机构
[1] Univ Fed Rio Grande do Sul UFRGS, Programa Posgrad Ciencias Farmaceut, BR-90610000 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Programa Posgrad Nanotecnol Farmaceut, BR-90610000 Porto Alegre, RS, Brazil
[3] Embrapa Suinos & Aves, BR-89715899 Concordia, Brazil
关键词
Curcumin; Lipid-core nanocapsules; Mucoadhesive; Oral squamous cell carcinoma (OSCC); Thermosensitive hydrogels; IN-SITU GEL; THERMOREVERSIBLE GEL; DRUG-DELIVERY; FORMULATION; VITRO; NANOPARTICLES; GROWTH;
D O I
10.1007/s13346-022-01227-1
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Buccal drug administration may be chosen as a medication route to treat various diseases for local or systemic effects. This study proposes the development of a thermosensitive hydrogel containing curcumin-loaded lipid-core nanocapsules coated with chitosan to increase mucoadhesion, circumventing several limitations of this route of administration. Hydroxy-propylmethylcellulose and Poloxamer (R) 407 were incorporated for hydrogel production. Physicochemical characterization parameters, such as particle size distribution, mean diameter, polydispersity index, zeta potential, and morphology, were analyzed. Spherical homogeneous particles were obtained with average diameter, of 173 +/- 22 nm for LNCc (curcumin lipidcore nanocapsules) and 179 +/- 48 nm for CLNCc (chitosan-curcumin lipid-core nanocapsules). A PDI equal to 0.09 +/- 0.02 for LNCc and 0.26 +/- 0.01 for CLNCc confirmed homogeneity. Tensile analysis and washability test on porcine buccal mucosa indicated higher mucoadhesion for hydrogels in comparison to the nanocapsules in suspension, remaining on the mucous membrane up to 8 h (10.92 +/- 3.95 mu g of curcumin washed for H-LNCc and 28.41 +/- 24.47 mu g for H-CLNCc) versus the latter, which remained washed on the membrane for 90 min only (62.60 +/- 4.72 mu g for LNCc and 52.08 +/- 1.63 mu g for CLNCc). The irritant potential (IR) of the formulations was evaluated by the hen's egg chorioallantoic membrane test (HET-CAM), with no irritation phenomena observed. Formulations were tested for their efficacy in an in vitro model against oral squamous cancer cell line, showing a significant reduction in cell viability on all tested groups. These findings demonstrated that the proposed nanosystem is mucoadhesive and has potential to deliver buccal treatments.
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页码:642 / 657
页数:16
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