Integrated metabolomics and network pharmacology revealed the key active ingredients for the treatment of ulcerative colitis in the Citrus reticulata 'Dahongpao' peel

被引：3

作者：

Li, Xue ^{[1
,2
]}

Wang, Qiaozhen ^{[1
,2
]}

Wang, Min ^{[1
,2
]}

Liu, Youping ^{[1
,2
]}

Chen, Lin ^{[1
,2
]}

Wang, Fu ^{[1
,2
]}

Chen, Hongping ^{[1
,2
]}

机构：

[1] Chengdu Univ Tradit Chinese Med, Dept Pharm, Standardizat Educ Minist Key Lab Tradit Chinese Me, Chengdu 611137, Sichuan, Peoples R China

[2] State Key Lab Southwestern Chinese Med Resource, Chengdu 611137, Sichuan, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2024年 / 239卷

基金：

中国国家自然科学基金;

关键词：

Active ingredients; Citrus reticulata pericarpium; Comparative metabolomics; Network pharmacology; Ulcerative colitis; INFLAMMATORY-BOWEL-DISEASE;

D O I：

10.1016/j.jpba.2023.115887

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Citrus reticulata pericarpium (CRP), the peel of Citrus reticulata 'Dahongpao' (DHP) is a medicinal herb with significant therapeutic value for treating ulcerative colitis (UC). However, the active therapeutic components of CRP are unclear. This study aims to reveal the metabolites potentially associated with the pharmacological properties of CRP. We performed flavonoid-targeting metabolomics to characterize the components of CRP (anti-UC part), tangerine pith and Citrus reticulata semen (no anti-UC effects parts) of DHP and further screened active components of CRP using network pharmacology and molecular docking. Lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were used to study the anti-inflammatory effect of the selected biologically active components. The therapeutic effects of the selected components were further investigated in a mouse model of UC induced by DSS. Three compounds, namely nobiletin, sinensetin, and hispidulin had the lowest docking scores among all screened ingredients. IL-6 and NO concentrations were significantly decreased in the LPS-stimulated RAW264.7 cells compared with control cells treated with these compounds. Moreover, UC mice treated with these com-pounds showed a reversal in weight loss, inhibition of shortening of colon length, and amelioration of colon injury. Our results indicated that sinensetin, nobiletin, and hispidulin can be potentially used for the treatment of UC.

引用

页数：11

共 42 条

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