Revealing the Structural Organization of Gamma-irradiated Starch Granules Using Polarization-resolved Second Harmonic Generation Microscopy

被引：4

作者：

Chen, Ming-Chi ^{[1
]}

Govindaraju, Indira ^{[2
]}

Wang, Wei-Hsun ^{[1
,3
]}

Chen, Wei-Liang

Mumbrekar, Kamalesh Dattaram ^{[4
]}

Mal, Sib Sankar ^{[5
]}

Sarmah, Bhaswati ^{[6
]}

Baruah, Vishwa Jyoti ^{[7
]}

Srisungsitthisunti, Pornsak ^{[8
]}

Karunakara, Naregundi ^{[9
,10
]}

Mazumder, Nirmal ^{[2
]}

Zhuo, Guan-Yu ^{[1
]}

机构：

[1] China Med Univ, Inst Translat Med & New Drug Dev, Coll Med, 91 Xueshi Rd, Taichung 404333, Taiwan

[2] Manipal Acad Higher Educ, Manipal Sch Life Sci, Dept Biophys, Planetarium complex, Manipal, Karnataka, India

[3] Natl Taiwan Univ, Ctr Condensed Matter Sci, 1, Sec 4, Roosevelt Rd, Taipei 106319, Taiwan

[4] Manipal Acad Higher Educ, Manipal Sch Life Sci, Dept Radiat Biol & Toxicol, Planetarium complex, Manipal, Karnataka, India

[5] Natl Inst Technol Karnataka, Dept Chem, Mat & Catalysis Lab, Surathkal 575025, Karnataka, India

[6] Assam Agr Univ, Dept Plant Breeding & Genet, Jorhat 785013, Assam, India

[7] Dibrugarh Univ, Dept Bioinformat, Dibrugarh 786004, Assam, India

[8] King Mongkuts Univ Technol North Bangkok, Fac Engn, Dept Prod Engn, Bangkok 10800, Thailand

[9] Mangalore Univ, Ctr Applicat Radioisotopes & Radiat Technol CARRT, Mangalore 574199, India

[10] Mangalore Univ, Ctr Adv Res Environm Radioact CARER, Mangalore 574199, India

来源：

MICROSCOPY AND MICROANALYSIS | 2023年 / 29卷 / 04期

关键词：

amylopectin; circular dichroism; polarization; second harmonic generation; second-order susceptibility; starch; CIRCULAR-DICHROISM; COLLAGEN; MYOSIN;

D O I：

10.1093/micmic/ozad058

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Starch is a semi-crystalline macromolecule with the presence of amorphous and crystalline components. The amorphous amylose and crystalline amylopectin regions in starch granules are susceptible to certain physical modifications, such as gamma irradiation. Polarization-resolved second harmonic generation (P-SHG) microscopy in conjunction with SHG-circular dichroism (CD) was used to assess the three-dimensional molecular order and inherent chirality of starch granules and their reaction to different dosages of gamma irradiation. For the first time, the relationship between starch achirality (chi(21)/chi(16) and chi(22)/chi(16)) and chirality (chi(14)/chi(16)) determining susceptibility tensor ratios has been elucidated. The results showed that changes in the structure and orientation of long-chain amylopectin were supported by the decrease in the SHG anisotropy factor and the chi(22)/chi(16) ratio. Furthermore, SHG-CD illustrated the molecular tilt angle by revealing the arrangement of amylopectin molecules pointing either upward or downward owing to molecular polarity.

引用

页码：1450 / 1459

页数：10

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