Potential biological active bacteriocin production by Bifidobacterium via eco-friendly, low-cost solid state fermentation principle

The present study is undertaken to evaluate the potential biological activities of bacteriocin produced by the Lactic acid bacteria Bifidobacterium sp. adopting solid state fermentation. Bifidobacterium was isolated from cooked fermented rice water. Isolated bacterial strain was identified by morphological, biochemical, and molecular characteristics. Sterilized cooked rice was inoculated with bacterial inoculum followed by extraction of bacteriocin under aseptic condition. Extracted bacteriocin was screened for antibacterial, antioxidant, and anti-inflammatory activities under invitro condition. Cooked fermentation rice medium used for solid state fermentation (SSF) supported growth and bacteriocin production by Bifidobacterium longum. Potential antibacterial activity of the extracted bacteriocin was confirmed by growth inhibition of Bacillus subtilis, and Escherichia coli strain. Notable free radical scavenging activity at 120 mu g/mL concentration of bacteriocin clearly indicated antioxidant activity. Anti-inflammatory activity of the bacteriocin was determined which showed maximum inhibition at 120 mu g/mL concentration. All these finding suggest the possible utilization of bacteriocin extraction from Bifidobacterium as an effective, safe bioactive agent.