Pre- and postsynaptic N-methyl-D-aspartate receptors are required for sequential printing of fear memory engrams

被引：6

作者：

Bertocchi, Ilaria ^{[1
,2
]}

Rocha-Almeida, Florbela ^{[3
]}

Romero-Barragan, Maria Teresa ^{[3
]}

Cambiaghi, Marco ^{[4
]}

Carretero-Guillen, Alejandro ^{[5
]}

Botta, Paolo ^{[6
]}

Dogbevia, Godwin K. ^{[1
,7
]}

Trevino, Mario ^{[1
,8
]}

Mele, Paolo ^{[2
]}

Oberto, Alessandra ^{[2
]}

Larkum, Matthew E. ^{[9
]}

Gruart, Agnes ^{[3
]}

Sprengel, Rolf ^{[1
]}

Delgado-Garcia, Jose Maria ^{[3
]}

Hasan, Mazahir T. ^{[1
,5
,10
]}

机构：

[1] Max Planck Inst Med Res, Dept Mol Neurobiol, Jahnstr 29, D-69120 Heidelberg, Germany

[2] Univ Turin, Neurosci Inst Cavalieri Ottolenghi NICO, Dept Neurosci Rita Levi Montalcini, I-10043 Turin, Italy

[3] Univ Pablo de Olavide, Div Neurosci, Ctra Utrera,Km 1, Seville 41013, Spain

[4] Univ Verona, Dept Neurosci Biomed & Movement Sci, Str Grazie 8, I-37134 Verona, Italy

[5] Achucarro Basque Ctr Neurosci, Lab Brain Circuits Therapeut, Sci Pk UPV-EHU,Sede Bldg,Barrio Sarriena S-N, Leioa 48940, Spain

[6] CNS drug Dev, Capital Reg Pharm, Copenhagen, Capital Region, Denmark

[7] Hlth Canada, 70 Colombine Driveway, Ottawa, ON K1A 0K9, Canada

[8] Univ Guadalajara, Lab Plast Cort & Aprendizaje Perceptual, Inst Neurociencias, Guadalajara, Mexico

[9] Charite, NeuroCure, Virchowweg 6, D-10117 Berlin, Germany

[10] Ikerbasque Basque Fdn Sci, Bilbao, Spain

来源：

ISCIENCE | 2023年 / 26卷 / 11期

关键词：

PRESYNAPTIC NMDA RECEPTORS; MEDIAL PREFRONTAL CORTEX; BASOLATERAL AMYGDALA; SYNAPTIC PLASTICITY; SYSTEMS CONSOLIDATION; NEURONAL CIRCUITS; EXPRESSION; REMOTE; OSCILLATIONS; NEUROSCIENCE;

D O I：

10.1016/j.isci.2023.108050

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The organization of fear memory involves the participation of multiple brain regions. However, it is largely unknown how fear memory is formed, which circuit pathways are used for "printing"memory engrams across brain regions, and the role of identified brain circuits in memory retrieval. With advanced genetic methods, we combinatorially blocked presynaptic output and manipulated N-methyl-D-aspartate receptor (NMDAR) in the basolateral amygdala (BLA) and medial prefrontal cortex (mPFC) before and after cued fear conditioning. Further, we tagged fear-activated neurons during associative learning for optogenetic memory recall. We found that presynaptic mPFC and postsynaptic BLA NMDARs are required for fear memory formation, but not expression. Our results provide strong evidence that NMDAR-dependent synaptic plasticity drives multi-trace systems consolidation for the sequential printing of fear memory engrams from BLA to mPFC and, subsequently, to the other regions, for flexible memory retrieval.

引用

页数：23

共 101 条

[81] Molecular and Cellular Approaches to Memory Allocation in Neural Circuits [J].