Mycoplasma pneumoniae carriage in children with recurrent respiratory tract infections is associated with a less diverse and altered microbiota

被引：22

作者：

Koenen, Mischa H. ^{[1
,5
]}

de Groot, Ruben C. A. ^{[2
]}

Piters, Wouter A. A. de Steenhuijsen ^{[3
,4
,5
]}

Chu, Mei Ling J. N. ^{[3
,5
]}

Arp, Kayleigh ^{[3
,5
]}

Hasrat, Raiza ^{[3
,5
]}

de Bruijn, Ad C. J. M. ^{[2
]}

Estevao, Silvia C. ^{[2
]}

Van der Vries, Erhard ^{[6
]}

Langereis, Jeroen D. ^{[7
]}

Boes, Marianne ^{[1
,5
]}

Bogaert, Debby ^{[4
,5
]}

van Rossum, Annemarie M. C. ^{[8
]}

Unger, Wendy W. J. ^{[2
]}

Verhagen, Lilly M. ^{[5
,7
,9
,10
]}

机构：

[1] UMC Utrecht, Ctr Translat Immunol, Utrecht, Netherlands

[2] Univ Med Ctr Rotterdam, Div Pediat Infect Dis & Immunol, Lab Pediat, Sophia Childrens Hosp,Erasmus MC, Rotterdam, Netherlands

[3] Natl Inst Publ Hlth & Environm, Ctr Infect Dis Control, Bilthoven, Netherlands

[4] Univ Edinburgh, Queens Med Res Inst, Ctr Inflammat Res, Edinburgh, Scotland

[5] Wilhelmina Childrens Hosp, Dept Pediat Infect Dis, Utrecht, Netherlands

[6] GD Anim Hlth, Dept Res & Dev, Deventer, Netherlands

[7] Radboud Univ Nijmegen, Dept Lab Med, Lab Med Immunol, Med Ctr,Sophia Childrens Hosp8, Nijmegen, Netherlands

[8] Univ Med Ctr, Dept Pediat, Div Pediat Infect Dis Immunol & Rheumatol, Erasmus MC, Rotterdam, Netherlands

[9] Radboud Univ Nijmegen, Amalia Childrens Hosp, Dept Pediat Infect Dis & Immunol, Med Ctr, Nijmegen, Netherlands

[10] Radboud Univ Nijmegen, Amalia Childrens Hosp, Dept Pediat Infect Dis & Immunol, Med Ctr, POB 9101,Geert Grootepl Zuid 32, NL-6500 HB Nijmegen, Netherlands

来源：

EBIOMEDICINE | 2023年 / 98卷

关键词：

STREPTOCOCCUS-PNEUMONIAE; TRANSMISSION; REVEALS; HEALTH; RISK;

D O I：

10.1016/j.ebiom.2023.104868

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background Mycoplasma pneumoniae is a common cause of community-acquired pneumonia in school-aged children and can be preceded by asymptomatic carriage. However, its role in recurrent respiratory tract infections is unclear. We studied the prevalence of M.pneumoniae carriage in children with recurrent respiratory infections and identified associated factors.Methods We tested M.pneumoniae carriage by qPCR in children with recurrent infections and their healthy family members in a cross-sectional study. Serum and mucosal total and M.pneumoniae-specific antibody levels were measured by ELISA and nasopharyngeal microbiota composition was characterized by 16S-rRNA sequencing.Findings Prevalence of M.pneumoniae carriage was higher in children with recurrent infections (68%) than their family members without infections (47% in siblings and 27% in parents). M.pneumoniae carriage among family members appeared to be associated with transmission within the household, likely originating from the affected child. In logistic regression corrected for age and multiple comparisons, IgA (OR 0.16 [0.06-0.37]) and total IgG deficiency (OR 0.15 [0.02-0.74]) were less prevalent in M.pneumoniae carriers (n = 78) compared to non-carriers (n = 36). In multivariable analysis, the nasopharyngeal microbiota of M.pneumoniae carriers had lower alpha diversity (OR 0.27 [0.09-0.67]) and a higher abundance of Haemophilus influenzae (OR 45.01 [2.74-1608.11]) compared to non-carriers. Interpretation M.pneumoniae carriage is highly prevalent in children with recurrent infections and carriers have a less diverse microbiota with an overrepresentation of disease-associated microbiota members compared to non-carriers. Given the high prevalence of M.pneumoniae carriage and the strong association with H. influenzae, we recommend appropriate antibiotic coverage of M.pneumoniae and H. influenzae in case of suspected pneumonia in children with recurrent respiratory tract infections or their family members.

引用

页数：14

共 58 条

[1] Low IgA Associated With Oropharyngeal Microbiota Changes and Lung Disease in Primary Antibody Deficiency [J].

Berbers, Roos-Marijn ;

Hoesein, Firdaus A. A. Mohamed ;

Ellerbroek, Pauline M. ;

van Montfrans, Joris M. ;

Dalm, Virgil A. S. H. ;

van Hagen, P. Martin ;

Paganelli, Fernanda L. ;

Viveen, Marco C. ;

Rogers, Malbert R. C. ;

de Jong, Pim A. ;

Uh, Hae-Won ;

Willems, Rob J. L. ;

Leavis, Helen L. .

FRONTIERS IN IMMUNOLOGY, 2020, 11

[2] Early Respiratory Microbiota Composition Determines Bacterial Succession Patterns and Respiratory Health in Children [J].

Biesbroek, Giske ;

Tsivtsivadze, Evgeni ;

Sanders, Elisabeth A. M. ;

Montijn, Roy ;

Veenhoven, Reinier H. ;

Keijser, Bart J. F. ;

Bogaert, Debby .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2014, 190 (11) :1283-1292

[3] Deep Sequencing Analyses of Low Density Microbial Communities: Working at the Boundary of Accurate Microbiota Detection [J].

Biesbroek, Giske ;

Sanders, Elisabeth A. M. ;

Roeselers, Guus ;

Wang, Xinhui ;

Caspers, Martien P. M. ;

Trzcinski, Krzysztof ;

Bogaert, Debby ;

Keijser, Bart J. F. .

PLOS ONE, 2012, 7 (03)

[4] Colonisation by Streptococcus pneumoniae and Staphylococcus aureus in healthy children [J].

Bogaert, D ;

van Belkum, A ;

Sluijter, M ;

Luijendijk, A ;

de Groot, R ;

Rümke, HC ;

Verbrugh, HA ;

Hermans, PWM .

LANCET, 2004, 363 (9424) :1871-1872

[5] Practice parameter for the diagnosis and management of primary immunodeficiency [J].

Bonilla, Francisco A. ;

Khan, David A. ;

Ballas, Zuhair K. ;

Chinen, Javier ;

Frank, Michael M. ;

Hsu, Joyce T. ;

Keller, Michael ;

Kobrynski, Lisa J. ;

Komarow, Hirsh D. ;

Mazer, Bruce ;

Nelson, Robert P., Jr. ;

Orange, Jordan S. ;

Routes, John M. ;

Shearer, William T. ;

Sorensen, Ricardo U. ;

Verbsky, James W. ;

Bernstein, David I. ;

Blessing-Moore, Joann ;

Lang, David ;

Nicklas, Richard A. ;

Oppenheimer, John ;

Portnoy, Jay M. ;

Randolph, Christopher R. ;

Schuller, Diane ;

Spector, Sheldon L. ;

Tilles, Stephen ;

Wallace, Dana .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2015, 136 (05) :1186-1205

[6] Maturation of the Infant Respiratory Microbiota, Environmental Drivers, and Health Consequences [J].

Bosch, Astrid A. T. M. ;

Piters, Wouter A. A. de Steenhuijsen ;

van Houten, Marlies A. ;

Chu, Mei Ling J. N. ;

Biesbroek, Giske ;

Kool, Jolanda ;

Pernet, Paula ;

de Groot, Pieter-Kees C. M. ;

Eijkemans, Marinus J. C. ;

Keijser, Bart J. F. ;

Sanders, Elisabeth A. M. ;

Bogaert, Debby .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2017, 196 (12) :1582-1590

[7] Coexistence of (partial) immune defects and risk of recurrent respiratory infections [J].

Bossuyt, Xavier ;

Moens, Leen ;

Van Hoeyveld, Erna ;

Jeurissen, Axel ;

Bogaert, Guy ;

Sauer, Kate ;

Proesmans, Marijke ;

Raes, Marc ;

De Boeck, Kris .

CLINICAL CHEMISTRY, 2007, 53 (01) :124-130

[8] Primary immunodeficiency in infection-prone children in southern Sweden: occurrence, clinical characteristics and immunological findings [J].

Brodszki, Nicholas ;

Jonsson, Goran ;

Skattum, Lillemor ;

Truedsson, Lennart .

BMC IMMUNOLOGY, 2014, 15

[9]

Callahan BJ, 2016, NAT METHODS, V13, P581, DOI [10.1038/NMETH.3869, 10.1038/nmeth.3869]

[10] The concordance between upper and lower respiratory microbiota in children with Mycoplasma pneumoniae pneumonia [J].

Dai, Wenkui ;

Wang, Heping ;

Zhou, Qian ;

Feng, Xin ;

Lu, Zhiwei ;

Li, Dongfang ;

Yang, Zhenyu ;

Liu, Yanhong ;

Li, Yinhu ;

Xie, Gan ;

Shen, Kunling ;

Yang, Yonghong ;

Zheng, Yuejie ;

Li, Shuaicheng .

EMERGING MICROBES & INFECTIONS, 2018, 7

← 1 2 3 4 5 6 →