Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus

被引：1

作者：

Ye, Qianyu ^{[1
,2
,3
]}

Wang, Jiali ^{[4
]}

Chen, Meijing ^{[5
]}

Nie, Weijian ^{[1
,2
,3
]}

Zhang, Huanxi ^{[1
,2
,3
]}

Su, Xiaojun ^{[1
,2
,3
]}

Ling, Liuting ^{[1
,2
,3
]}

Liu, Xiangjun ^{[6
]}

Liu, Longshan ^{[1
,2
,3
,7
]}

Wang, Changxi ^{[1
,2
,3
,7
]}

Gao, Yifang ^{[1
,2
,3
,7
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 1, Organ Transplantat Ctr, Guangzhou, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 1, Guangdong Prov Key Lab Organ Donat & Transplant Im, Guangzhou, Peoples R China

[3] Sun Yat Sen Univ, Affiliated Hosp 1, Guangdong Prov Int Cooperat Base Sci & Technol Org, Guangzhou, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Nephrol, Guangzhou, Peoples R China

[5] Guangzhou BFR Gene Dignost, Guangzhou, Peoples R China

[6] Beijing BFR Gene Diagnost, Beijing, Peoples R China

[7] Sun Yat Sen Univ, Affiliated Hosp 1, Organ Transplantat Ctr, Guangdong Prov Key Lab Organ Donat & Transplant Im, 58,Zhong Shan 2nd St, Guangzhou 510080, Peoples R China

来源：

HELIYON | 2023年 / 9卷 / 06期

关键词：

FlowSpot; ELISpot; Serologic analysis; CMV-Specific T-cells; IFN-& gamma; TRANSPLANT RECIPIENTS; INFECTION; ELISPOT; CYTOMETRY; CD4(+); DRIVEN; VIRUS;

D O I：

10.1016/j.heliyon.2023.e16792

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Objectives: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-?). CMV-specific, T-cell-released IFN-? was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell response in healthy individuals. The FlowSpot results were compared with those of serological analysis and enzyme-linked immunospot (ELISpot) assay. Methods: Experimental results and parameter analysis were investigated by using serological, ELISpot, and FlowSpot assays. Results: The levels of IFN-?, which is released from CMV-specific T-cells, were measured, and the results and parameter analysis showed a good correlation between FlowSpot and ELISpot. However, FlowSpot was more sensitive and better reflected the strength of IFN-? secretion than did ELISpot.Conclusions: Compared to ELISpot, FlowSpot has a high sensitivity and is cost and time effective. Thus, this method can be used in wider clinical and scientific applications.

引用

页数：9

共 50 条

[1] INTERFERON-GAMMA (IFN-GAMMA) REGULATES T-CELL RESPONSE TO MELANOMA
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[10] IMMUNOGENIZATION OF A MURINE T-CELL LYMPHOMA VIA TRANSFECTION WITH INTERFERON-GAMMA
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