DNA Fragment Enrichment for High-Throughput Sequencing

被引：1
|
作者：
Sinyakov, A. N. [1 ]
Kostina, E. V. [1 ]
机构：
[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Branch, Novosibirsk 630090, Russia
来源：
MOLECULAR BIOLOGY | 2023年 / 57卷 / 03期
关键词：
high-throughput sequencing; oligonucleotides; microarray; amplification; diagnostics; exome; CRISPR-Cas; TARGET-ENRICHMENT; HYBRID SELECTION; GENERATION; CAPTURE; GENOME; PCR; MUTATION; OLIGONUCLEOTIDES; AMPLIFICATION; CARCINOMA;
D O I：
10.1134/S002689332303010X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This review describes the application of oligonucleotides, which are mainly obtained using DNA synthesizers of a new generation (microarray DNA synthesizers), for the enrichment of target genomic fragments. The methods of molecular hybridization, polymerase chain reaction, and CRISPR-Cas9 system for this purpose are considered. Examples of the practical use of the developed methods for research and diagnostic purposes are given.
引用
收藏
页码：424 / 439
页数：16
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