A low-cost and hand-hold PCR microdevice based on water-cooling technology

被引:1
作者
Sun, Kaixin [1 ]
Whiteside, Ben [2 ]
Hebda, Michael [2 ]
Fan, Yiqiang [1 ]
Zhang, Yajun [1 ]
Xie, Yumeng [1 ]
Liang, KunMing [3 ]
机构
[1] Beijing Univ Chem Technol, Sch Mech & Elect Engn, Beijing, Peoples R China
[2] Univ Bradford, Fac Engn & informat, Bradford, England
[3] LK Inject Molding Machine Co LTD, Guangdong, Peoples R China
关键词
PCR microdevice; Nucleic acid amplification; Water-cooling; Point-of-care testing; CHIP; SYSTEM;
D O I
10.1007/s10544-023-00652-4
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Polymerase chain reaction (PCR) has become a powerful tool for detecting various diseases due to its high sensitivity and specificity. However, the long thermocycling time and the bulky system have limited the application of PCR devices in Point-of-care testing. Herein, we have proposed an efficient, low-cost, and hand-hold PCR microdevice, mainly including a control module based on water-cooling technology and an amplification module fabricated by 3D printing. The whole device is tiny and can be easily hand-held with a size of about 110 mm x 100 mm x 40 mm and a weight of about 300 g at a low cost of about $170.83. Based on the water-cooling technology, the device can efficiently perform 30 thermal cycles within 46 min at a heating/cooling rate of 4.0/8.1 celcius/s. To test our instrument, plasmid DNA dilutions were amplified with this device; the results demonstrate successful nucleic acid amplification of the plasmid DNA and exhibit the promise of this device for Point-of-care testing.
引用
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页数:7
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