NF-κB p65 and TCF-4 interactions are associated with LPS-stimulated IL-6 secretion of macrophages

被引：4

作者：

Kim, Ji-Youn ^{[1
]}

Jee, Hyeon-Gun ^{[1
]}

Kim, Ju Yeong ^{[2
]}

Yong, Tai-Soon ^{[2
]}

Jeon, Soung-Hoo ^{[2
]}

机构：

[1] Catholic Univ Korea, Coll Med, Dept Dent, Div Oral & Maxillofacial Surg,St Vincents Hosp, Seoul 06591, South Korea

[2] Yonsei Univ, Coll Med, Dept Trop Med, Seoul 03722, South Korea

来源：

BIOCHEMISTRY AND BIOPHYSICS REPORTS | 2024年 / 38卷

关键词：

Macrophage; Inflammatory cytokine; Lipopolysaccharides; NF; kappa B signaling pathway; Wnt/ beta-catenin signaling pathway; CYTOKINE EXPRESSION; INTERLEUKIN-6; ACTIVATION;

D O I：

10.1016/j.bbrep.2024.101659

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proinflammatory cytokine plays a central role in host defense and acute inflammatory responses. Both positive and negative correlations of NF-kappa B and Wnt/beta-catenin pathways have been reported depending on cell types in response to inflammatory stimuli for IL -6 cytokine production. Macrophages are vital to the regulation of immune responses and the development of inflammation, but the crosstalk between two pathways has not been elucidated so far in macrophages. We observed a positive cross-regulation between the NF-kappa B and Wnt/beta-catenin pathways for IL -6 production in human macrophages. To verify the functional validity of this interaction, LY294002 or PNU74654, representative blockers of each pathway, were treated. IL -6 secretion was reduced to the basal level by both inhibitor treatments, even when stimulated by LPS. We also found that NF-kappa B p65 migrated to the nucleus and interacted with the transcription factor TCF-4 in macrophages upon LPS stimulation.

引用

页数：7

共 30 条

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