Antiviral effects of duck type I and type III interferons against Duck Tembusu virus in vitro and in vivo

Duck Tembusu Virus (DTMUV) is a newly emerging avian flavivirus that causes substantial economic losses to the duck industry in Asia by causing severe egg drop syndrome and fatal encephalitis in domestic ducks. During viral replication, host cells recognize the RNA structures produced by DTMUV, which triggers the production of interferons (IFNs) to inhibit viral replication. However, the function of duck type I and type III IFNs in inhibiting DTMUV infection remains largely unknown. In this study, we expressed and purified recombinant duck IFN-beta (duIFN-beta) and IFN-lambda (duIFN-lambda) in Escherichia coli and evaluated their antiviral activity against vesicular stomatitis virus (VSV). Furthermore, we found that both duIFN-beta and duIFN-lambda activated the ISRE promoter and induced the expression of ZAP, OAS, and RNaseL in duck embryo fibroblasts (DEFs). Notably, duIFN-beta showed faster and more potent induction of ISGs in vitro and in vivo compared to duIFN-lambda. Moreover, both duIFN-beta and duIFN-lambda showed high potential to inhibit DTMUV infection in DEFs, with duIFN-beta demonstrating better antiviral efficacy than duIFN-lambda against DTMUV in ducks. In conclusion, our results revealed that both duIFN-beta and duIFN-lambda can induce ISGs production and exhibit significant antiviral activity against DTMUV in vitro and in vivo, providing new insights for the development of antiviral therapeutic strategies in ducks.