Recent progress in rational design of peroxynitrite fluorescent probes and bioapplication

Peroxynitrite (ONOO-), a reactive oxygen species, is extensively found in organisms. It is strongly associated with many diseases, serving as a significant role in physiological and pathological processes. As a result, real-time and in situ detecting of ONOO- is important for the in-depth study of its biological functions. In recent years, the fluorescence detection technology has been widely used in the detection of ONOO-. In this review, we sum-marized and classified ONOO- fluorescent probes according to different interaction mechanisms, and we introduced their performance and applications in biological imaging. The specific challenges and future devel-opment prospects of ONOO- fluorescent probes are also concerned here. We expect that this review not only provides a reference for people who are just beginning to learn, but also provides an overall reference idea for developing a new generation of ONOO- fluorescent probes, promoting its widespread application in practice.