Development of a Lateral-Flow Immunochromatographic Strip for the Detection of Oxytetracycline Residues in Biological Fluids

被引:4
|
作者
Aleem, Abdul [1 ]
Akhtar, Bushra [2 ]
Muhammad, Faqir [3 ]
Qureshi, Anas Sarwar [4 ]
Rahman, Sajjad-ur [5 ]
机构
[1] Univ Agr Faisalabad, Inst Physiol & Pharmacol, Faisalabad 38000, Pakistan
[2] Univ Agr Faisalabad, Dept Pharm, Faisalabad 38000, Pakistan
[3] Bahauddin Zakariya Univ, Dept Biosci, Fac Vet Sci, Multan 60800, Pakistan
[4] Univ Agr Faisalabad, Dept Anat, Faisalabad 38000, Pakistan
[5] Univ Agr Faisalabad, Inst Microbiol, Faisalabad 38000, Pakistan
来源
ACS OMEGA | 2023年 / 8卷 / 39期
关键词
POLYCLONAL ANTIBODY; MILK; ASSAY; PURIFICATION;
D O I
10.1021/acsomega.3c04759
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Oxytetracycline (OTC) is extensively used in veterinary medicine and for growth promotion around the globe. The indiscriminate use of OTC in food-producing animals leaves residues in animal products. The presence of these residues in animal products causes economic losses and harmful effects on consumers. Different regulatory bodies set maximum residue limits (MRLs) for different tetracyclines. To avoid harmful effects, there is a need for a simple, fast, and economical method for the screening of animal products. In this study, a fast, economical, and user-friendly lateral-flow immunochromatographic (LFIC) assay based on gold nanoparticles (AuNPs) was developed to detect the presence of OTC residues in biological fluids. AuNPs provided visual results as red lines in 6-15 min. Polyclonal rabbit IgG antibodies were produced using the immunogen of OTC. These antibodies were purified by the combined ammonium sulfate-octanoic acid precipitation method. Antibodies were conjugated to AuNPs as recognition biomolecules. A LFIC strip was optimized using borate buffer spiked with different concentrations of the OTC. The visual limit of detection (LOD) in different biological samples (milk, serum, and urine) was determined using samples spiked with OTC. The LOD was found to be 15 mu g/L, which is very low from the MRL (100 mu g/L) set by different regulatory authorities. This LFIC strip can be used to detect OTC residues in biological fluids for point-of-care testing (POCT). These strips are easy to use, cost-effective, and portable and provide quick results without the use of laboratory instruments.
引用
收藏
页码:36237 / 36244
页数:8
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