Bioactive Conjugated Polymer-Based Biodegradable 3D Bionic Scaffolds for Facilitating Bone Defect Repair

Bone defect regeneration is one of the great clinical challenges. Suitable bioactive composite scaffolds with high biocompatibility, robust new-bone formation capability and degradability are still required. This work designs and synthesizes an unprecedented bioactive conjugated polymer PT-C3-NH2, demonstrating low cytotoxicity, cell proliferation/migration-promoting effect, as well as inducing cell differentiation, namely regulating angiogenesis and osteogenesis to MC3T3-E1 cells. PT-C3-NH2 is incorporated into polylactic acid-glycolic acid (PLGA) scaffolds, which is decorated with caffeic acid (CA)-modified gelatin (Gel), aiming to improve the surface water-wettability of PLGA and also facilitate to the linkage of conjugated polymer through catechol chemistry. A 3D composite scaffold PLGA@GC-PT is then generated. This scaffold demonstrates excellent bionic structures with pore size of 50-300 mu m and feasible biodegradation ability. Moreover, it also exhibites robust osteogenic effect to promote osteoblast proliferation and differentiation in vitro, thus enabling the rapid regeneration of bone defects in vivo. Overall, this study provides a new bioactive factor and feasible fabrication approach of biomimetic scaffold for bone regeneration. This work synthesizes a novel conjugated polymer, PT-C3-NH2, and combines it with a biodegradable porous scaffold to construct a 3D composite porous scaffold, PLGA@GC-PT. Bioactive PT-C3-NH2 with excellent biocompatibility promotes endothelial cell migration and induces osteoblast differentiation. The PLGA@GC-PT scaffold shows great biomimetic properties and biodegradability, and exhibits excellent new bone generation in vitro and in vivo.image