Streptococcus sciuri sp. nov., Staphylococcus marylandisciuri sp. nov. and Staphylococcus americanisciuri sp. nov., isolated from faeces of eastern grey squirrel (Sciurus carolinensis)

被引：1

作者：

Volokhov, Dmitriy V. ^{[1
]}

Zagorodnyaya, Tatiana A. ^{[1
]}

Furtak, Vyacheslav A. ^{[1
]}

Nattanmai, Geetha ^{[2
]}

Randall, Linnell ^{[2
]}

Jose, Sherly ^{[2
]}

Gao, Yamei ^{[1
]}

Eisenberg, Tobias ^{[3
]}

Delmonte, Pierluigi ^{[4
]}

Blom, Jochen ^{[5
]}

Mitchell, Kara K. ^{[2
]}

机构：

[1] US FDA, Ctr Biol Evaluat & Res, 10903 New Hampshire Ave, Silver Spring, MD 20993 USA

[2] New York State Dept Hlth, Wadsworth Ctr, Bacteriol Lab, 120 New Scotland Ave, Albany, NY 12208 USA

[3] Hessian State Lab LHL, Dept Vet Med, Schubertstr 60, D-35392 Giessen, Germany

[4] US FDA, Ctr Food Safety & Appl Nutr, 5001 Campus Dr, College Pk, MD 20740 USA

[5] Justus Liebig Univ Giessen, Bioinformat & Syst Biol, Heinrich Buff Ring 58, D-35392 Giessen, Germany

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2023年 / 73卷 / 08期

关键词：

Streptococcus; Staphylococcus; squirrel; phylogeny; 16S rRNA; genomics; 16S RIBOSOMAL-RNA; PROKARYOTIC TAXONOMY; GENE; IDENTIFICATION; CLASSIFICATION; SEPARATION; RPOB; SODA; SET; ERA;

D O I：

10.1099/ijsem.0.006015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

One novel Streptococcus strain (SQ9- PEAT) and two novel Staphylococcus strains (SQ8- PEAT and GRT3T) were isolated from faeces of a wild eastern grey squirrel. The strains were non- spore-forming, non-motile Gram-positive cocci, facultative anaer-obes. The genomes for these strains were sequenced. The 16S rRNA gene and core-genome- based phylogenetic analyses showed that strain SQ9- PEAT was closely related to Streptococcus hyointestinalis, strain SQ8- PEAT to Staphylococcus petten-koferi and Staphylococcus argensis, and strain GRT3T to Staphylococcus rostri, Staphylococcus muscae and Staphylococcus microti. Average nucleotide identity and pairwise digital DNA-DNA hybridization values calculated for these novel strains compared to type strain genomes of phylogenetically related species within the genera Streptococcus and Staphylococcus clearly revealed that strain SQ9- PEAT represents a novel species of the genus Streptococcus and strains SQ8- PEAT and GRT3T represent two novel species of the genus Staphylococcus. Phenotypical features of these novel type strains differed from the features of the type strains of other phylogenetically related species. MALDI- TOF mass spectrometry supported identification of these novel species. Based on these data, we propose one novel species of the genus Streptococcus, for which the name Streptococcus sciuri sp. nov. with the type strain SQ9- PEAT (=DSM 114656T=CCUG 76426T=NCTC 14727T) is proposed, and two novel species of the genus Staphylococcus, for which the names Staphylococcus marylandisciuri sp. nov. with the type strain SQ8-PEAT (=DSM 114685T=CCUG 76423T=NCTC 14723T) and Staphylococcus americanisciuri sp. nov. with the type strain GRT3T (=DSM 114696T=CCUG 76427T=NCTC 14722T) are proposed. The genome G+C contents are 38.29, 36.49 and 37.26 mol% and complete draft genome sizes are 1 692 266, 2 371 088 and 2 237 001 bp for strains SQ9- PEAT, SQ8-PEAT and GRT3T, respectively.

引用

页数：13

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