Targeted proteomic analysis reveals that crocodile oil from Crocodylus siamensis may enhance hepatic energy metabolism in rats

被引:1
作者
Fungfuang, Wirasak [1 ,2 ]
Srisuksai, Krittika [2 ]
Santativongchai, Pitchaya [3 ]
Charoenlappanit, Sawanya [4 ]
Phaonakrop, Narumon [4 ]
Roytrakul, Sittiruk [4 ]
Tulayakul, Phitsanu [5 ]
Parunyakul, Kongphop [2 ]
机构
[1] Kasetsart Univ, Kasetsart Univ Res & Dev Inst, Ngamwongwan Rd, Bangkok 10900, Thailand
[2] Kasetsart Univ, Fac Sci, Dept Zool, Ngamwongwan Rd, Bangkok 10900, Thailand
[3] Kasetsart Univ, Fac Vet Med, Int Program, Biovet Sci, Ngamwongwan Rd, Bangkok 10900, Thailand
[4] Natl Sci & Technol Dev Agcy, Funct Ingredients & Food Innovat Res Grp, Natl Ctr Genet Engn & Biotechnol BIOTEC, Phahonyothin Rd, Khlong Luang 12120, Pathum Thani, Thailand
[5] Kasetsart Univ, Fac Vet Med, Dept Vet Publ Hlth, Malaiman Rd, Kamphaeng Saen 73140, Nakhon Pathom, Thailand
关键词
crocodile oil; energy metabolism; liver; proteomics; rat; OXIDATIVE-PHOSPHORYLATION; PALM OIL; DIET; FAT; LACTATE; IDENTIFICATION; QUANTITATION; INFLAMMATION; SENSITIVITY; ACTIVATION;
D O I
10.1538/expanim.23-0009
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The liver is a key organ governing body energy metabolism. Dietary fats influence energy metabolism and mitochondrial functioning. Crocodile oil (CO) is rich in mono-and polyunsaturated fatty acids that contain natural anti-inflammatory and healing properties. Our study examined how CO affects the expressions of liver proteins involved in energy metabolism in rats. Twenty-one male Sprague Dawley rats were divided into three groups and underwent oral gavage with 3 ml/kg of sterile water (N group), CO (CO group), or palm oil (PO group) for 7 weeks. Body weight, energy intake, liver weight, liver indexes, blood lipid profiles, and liver-energy intermediates were measured. The liver proteome was analyzed using shotgun proteomics, and the functions and network interactions of several candidate proteins were predicted using the STITCH v.5.0 software. Body weights, energy intake, liver contents, and lipid profiles did not differ between the groups. However, hepatic oxaloacetate and malate levels were significantly higher in the CO group than in the PO group. Targeted proteomics reveals that 22 out of 1,790 unique proteins in the CO group were involved in energy-generating pathways, including the tricarboxylic acid cycle and oxidative phosphorylation (OXPHOS), and were correlated with the AMP-activated protein kinase signaling pathway. Cluster analysis of 59 differentially expressed proteins showed that OXPHOS-associated proteins were upregulated in the CO group and that three glycolytic metabolism-related proteins were downregulated in the CO group. CO may enhance hepatic energy metabolism by regulating the expressions of energy expenditure related proteins.
引用
收藏
页码:425 / 438
页数:14
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