Gut microbiota signatures and fecal metabolites in postmenopausal women with osteoporosis

被引：22

作者：

Wang, Han ^{[1
,2
]}

Liu, Jing ^{[3
]}

Wu, Zuoxing ^{[1
,2
]}

Zhao, Yangyang ^{[3
]}

Cao, Man ^{[4
]}

Shi, Baohong ^{[1
,2
]}

Chen, Baolong ^{[4
]}

Chen, Ning ^{[5
]}

Guo, Hao ^{[6
]}

Li, Na ^{[1
,2
]}

Chen, Jian ^{[3
]}

Xu, Ren ^{[1
,2
]}

机构：

[1] Fac Med & Life Sci, Sch Med, State Key Lab Cellular Stress Biol, 4221 Xiangan South Rd, Xiamen 361102, Fujian, Peoples R China

[2] Xiamen Univ, Affiliated Hosp 1, ICMRS Collaborating Ctr Skeletal Stem Cells, Xiamen Key Lab Regenerat Med,Fujian Prov Key Lab O, Xiamen 361005, Peoples R China

[3] Xiamen Univ, Zhongshan Hosp, Dept Rehabil, 201-209 Hubinnan Rd, Xiamen 361000, Fujian, Peoples R China

[4] Xiamen Treatgut Biotechnol Co Ltd, Xiamen 361001, Fujian, Peoples R China

[5] Fudan Univ, Zhongshan Hosp Xiamen, Dept Endocrinol, Xiamen 361000, Peoples R China

[6] Xiamen Univ, Fac Med & Life Sci, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen 361102, Peoples R China

来源：

GUT PATHOGENS | 2023年 / 15卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Postmenopausal women; Gut microbiota; Fecal metabolites; Osteoporosis; ALPHA-LINOLENIC ACID; BONE LOSS; MENOPAUSE;

D O I：

10.1186/s13099-023-00553-0

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

BackgroundWomen suffer from various distress and disturbances after menopause, including osteoporosis, a risk factor associated with multiple diseases. Altered gut microbiota has been implicated in postmenopausal osteoporosis. In this study, to understand gut microbiota signatures and fecal metabolite changes in postmenopausal women with osteoporosis, 108 postmenopausal women were recruited for intestinal microbiota and fecal metabolite detection. Among these participants, 98 patients, who met the inclusion criteria, were divided into postmenopausal osteoporosis (PMO) and non-postmenopausal osteoporosis (non-PMO) groups based on bone mineral density (BMD). The compositions of gut bacteria and fungi were examined by 16 S rRNA gene sequencing and ITS sequencing, respectively. Meanwhile, fecal metabolites were analyzed using liquid chromatography coupled with mass spectrometry (LC-MS).ResultsWe found that bacterial & alpha;-diversity and & beta;-diversity were significantly altered in PMO compared to non-PMO patients. Interestingly, fungi composition showed larger changes, and the differences in & beta;-diversity were more significant between PMO and non-PMO patients. Metabolomics analysis revealed that fecal metabolites, such as levulinic acid, N-Acetylneuraminic acid, and the corresponding signaling pathways were also changed significantly, especially in the alpha-Linolenic acid metabolism and selenocompound metabolism. The screened differential bacteria, fungi, and metabolites closely correlated with clinical findings between these two groups, for example, the bacterial genus, Fusobacterium, the fungal genus, Devriesia, and the metabolite, L-pipecolic acid, were significantly associated with BMD.ConclusionsOur findings indicated that there were remarkable changes in gut bacteria, fungi, and fecal metabolites in postmenopausal women, and such changes were notably correlated with patients' BMD and clinical findings. These correlations provide novel insights into the mechanism of PMO development, potential early diagnostic indicators, and new therapeutic approaches to improve bone health in postmenopausal women.

引用

页数：13