Application of Novel Short Tandem Repeat Typing for Wickerhamomyces anomalus Reveals Simultaneous Outbreaks within a Single Hospital

被引:6
作者
Spruijtenburg, Bram [1 ,2 ]
Rudramurthy, Shivaprakash M. [3 ]
Meijer, Eelco F. J. [1 ,2 ]
van Haren, Merlijn H. I. [1 ]
Kaur, Harsimran [3 ]
Chakrabarti, Arunaloke [3 ]
Meis, Jacques F. [1 ,2 ,4 ]
de Groot, Theun [1 ,2 ]
机构
[1] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, NL-6532 SZ Nijmegen, Netherlands
[2] Radboud Univ Nijmegen Med Ctr, Canisius Wilhelmina Hosp, Ctr Expertise Mycol, NL-6532 SZ Nijmegen, Netherlands
[3] Postgrad Inst Med Educ & Res, Dept Med Microbiol, Chandigarh 160012, India
[4] Univ Cologne, Excellence Ctr Med Mycol, Dept Internal Med 1, D-50931 Cologne, Germany
关键词
Wickerhamomyces anomalus; Candida pelliculosa; short tandem repeats; genotyping; whole-genome sequencing; antifungal susceptibility testing; outbreak investigation; CANDIDA-PELLICULOSA FUNGEMIA; BLOOD-STREAM INFECTION; PICHIA-ANOMALA; ANTIFUNGAL RESISTANCE; AZOLE RESISTANCE; HETEROZYGOSITY; MUTATIONS; IDENTIFICATION; DATABASE; ALBICANS;
D O I
10.3390/microorganisms11061525
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Wickerhamomyces anomalus, previously known as Candida pelliculosa, occasionally causes candidemia in humans, primarily infecting neonates, and infants. The mortality rate of these invasive infections is high, and isolates with a reduced susceptibility to fluconazole have been reported. W. anomalus outbreaks are regularly reported in healthcare facilities, especially in neonatal intensive care units (NICUs). In order to rapidly genotype isolates with a high-resolution, we developed and applied a short tandem repeat (STR) typing scheme for W. anomalus. Six STR markers were selected and amplified in two multiplex PCRs, M3 and M6, respectively. In total, 90 W. anomalus isolates were typed, leading to the identification of 38 different genotypes. Four large clusters were found, unveiling simultaneous outbreak events spread across multiple units within the same hospital. STR typing results of 11 isolates were compared to whole-genome sequencing (WGS) single nucleotide polymorphism (SNP) calling, and the identified genotypic relationships were highly concordant. We performed antifungal susceptibility testing of these isolates, and a reduced susceptibility to fluconazole was found for two (2.3%) isolates. ERG11 genes of these two isolates were examined using WGS data, which revealed a novel I469L substitution in one isolate. By constructing a homology model for W. anomalus ERG11p, the substitution was found in close proximity to the fluconazole binding site. In summary, we showed multiple W. anomalus outbreak events by applying a novel STR genotyping scheme.
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页数:14
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