A large Stokes shift NIR fluorescent probe for visual monitoring of mitochondrial peroxynitrite during inflammation and ferroptosis and in an Alzheimer's disease model

被引：15

作者：

Chen, Shiying ^{[1
]}

Huang, Wei ^{[1
]}

Tan, Hongli ^{[1
]}

Yin, Guoxing ^{[1
]}

Chen, Shengyou ^{[1
]}

Zhao, Kuicheng ^{[1
]}

Huang, Yinghui ^{[1
]}

Zhang, Youyu ^{[1
]}

Li, Haitao ^{[1
]}

Wu, Cuiyan ^{[1
,2
]}

机构：

[1] Hunan Normal Univ, Coll Chem & Chem Engn, Changsha 410081, Peoples R China

[2] Ningbo Univ Technol, Sch Mat & Chem Engn, Ningbo 315211, Peoples R China

来源：

ANALYST | 2023年 / 148卷 / 18期

基金：

中国国家自然科学基金;

关键词：

NEAR-INFRARED FLUORESCENT; NITRIC-OXIDE; IN-VITRO; SELECTIVE DETECTION; SUPEROXIDE; BIOLOGY; DAMAGE; FORM; DNA;

D O I：

10.1039/d3an00956d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The excessive formation of peroxynitrite (ONOO-) in mitochondria has been implicated in various pathophysiological processes and diseases. However, owing to short emission wavelengths and small Stokes shifts, previously reported fluorescent probes pose significant challenges for mitochondrial ONOO- imaging in biological systems. In this study, a near-infrared (NIR) fluorescent probe, denoted as DCO-POT, is designed for the visual monitoring of mitochondrial ONOO-, displaying a remarkable Stokes shift of 170 nm. The NIR fluorophore of DCO-CHO is released by DCO-POT upon the addition of ONOO-, resulting in off-on NIR fluorescence at 670 nm. This phenomenon facilitates the high-resolution confocal laser scanning imaging of ONOO- generated in biological systems. The practical applications of DCO-POT as an efficient fluorescence imaging tool are verified in this study. DCO-POT enables the fluorometric visualization of ONOO- in organelles, cells, and organisms. In particular, ONOO- generation is analyzed during cellular and organism-level (zebrafish) inflammation during ferroptosis and in an Alzheimer's disease mouse model. The excellent visual monitoring performance of DCO-POT in vivo makes it a promising tool for exploring the pathophysiological effects of ONOO-.

引用

页码：4331 / 4338

页数：8

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