A self-assembled nanoparticle vaccine based on pseudorabies virus glycoprotein D induces potent protective immunity against pseudorabies virus infection

被引：13

作者：

Ren, Xujiao ^{[1
,2
]}

Cao, Nan ^{[1
,2
]}

Tian, Linxing ^{[1
,2
]}

Liu, Wenqiang ^{[1
,2
]}

Zhu, Hechao ^{[1
,2
]}

Rong, Zhenxiang ^{[1
,2
]}

Yao, Manman ^{[1
,2
]}

Li, Xiangmin ^{[1
,2
,3
]}

Qian, Ping ^{[1
,2
,3
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China

[2] Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China

[3] Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan 430070, Hubei, Peoples R China

来源：

VETERINARY MICROBIOLOGY | 2023年 / 284卷

关键词：

Pseudorabies virus; Glycoprotein D; Nanoparticle vaccine; Lumazine synthase; Cellular immunity; AUJESZKYS-DISEASE; GROWING PIGS; VARIANT; DNA;

D O I：

10.1016/j.vetmic.2023.109799

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pseudorabies virus (PRV) mainly causes pseudorabies (PR) or Aujeszky's disease in pigs and can infect humans, raising public health concerns about zoonotic and interspecies transmission of PR. With the emergence of PRV variants in 2011, the classic attenuated PRV vaccine strains have failed to protect many swine herds against PR. Herein, we developed a self-assembled nanoparticle vaccine that induces potent protective immunity against PRV infection. PRV glycoprotein D (gD) was expressed using the baculovirus expression system and further presented on the lumazine synthase (LS) 60-meric protein scaffolds via the SpyTag003/SpyCatcher003 covalent coupling system. In mouse and piglet models, LSgD nanoparticles emulsified with the ISA 201VG adjuvant elicited robust humoral and cellular immune responses. Furthermore, LSgD nanoparticles provided effective protection against PRV infection and eliminated pathological symptoms in the brain and lungs. Collectively, the gDbased nanoparticle vaccine design appears to be a promising candidate for potent protection against PRV infection.

引用

页数：15

共 39 条

[1] Pseudorabies Virus Variant in Bartha-K61-Vaccinated Pigs, China, 2012 [J].