Transcriptomic Profiling of Pleural Effusions: Differences in Malignant and Infectious Fluids

被引:0
|
作者
Zamora-Molina, Lucia [1 ,2 ]
Garcia-Pachon, Eduardo [1 ]
Amoros, Marta [3 ]
Gijon-Martinez, Julia [3 ]
Sanchez-Almendro, Judith [3 ]
Baeza-Martinez, Carlos [1 ,2 ]
Hernandez-Blasco, Luis [2 ]
Galiana, Antonio [3 ]
机构
[1] Hosp Gen Univ Elche, Sect Resp Med, Elche 03203, Alicante, Spain
[2] Univ Miguel Hernandez Elche, Dept Clin Med, Elche 03202, Alicante, Spain
[3] Hosp Gen Univ Elche, Dept Microbiol, FISABIO, Elche 03203, Alicante, Spain
来源
MEDICINA-LITHUANIA | 2024年 / 60卷 / 03期
关键词
biomarkers; high-throughput sequencing; inflammation; mRNA; pleural effusion; transcriptome; MESSENGER-RNA; EXPRESSION; QUANTIFICATION;
D O I
10.3390/medicina60030424
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background and Objectives: Different cellular and molecular processes are involved in the production of malignant and infectious pleural effusions. However, the underlying mechanisms responsible for these differences or their consequences remain incompletely understood. The objective of this study was to identify differences in gene expression in pleural exudates of malignant and infectious aetiology and establish the possible different biological processes involved in both situations. Materials and Methods: RNA transcriptomic analysis was performed on 46 pleural fluid samples obtained during diagnostic thoracocenteses from 46 patients. There were 35 exudates (19 malignant and 16 infectious effusions) and 11 transudates that were used as a reference control group. Differential gene expression analysis for both exudative groups was identified. An enrichment score using the Human Kegg Orthology database was used for establishing the biological processes associated with malignant and infectious pleural effusions. Results: When comparing malignant exudates with infectious effusions, 27 differentially expressed genes with statistical significance were identified. Network analysis showed ten different biological processes for malignant and for infectious pleural effusions. In malignant fluids, processes related to protein synthesis and processing predominate. In infectious exudates, biological processes in connection with ATP production prevail. Conclusions: This study demonstrates differentially expressed genes in malignant and infectious pleural effusions, which could have important implications in the search for diagnostic or prognostic biomarkers. In addition, for the first time, biological processes involved in these two causes of pleural exudates have been described.
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页数:14
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