The effects of allogeneic and xenogeneic lyophilized leukocyte-and platelet-rich fibrin on bone healing in rat

Background: Critical size defects are one of the challenges in the treatment of fractures in humans and animals. Blood products such as leukocyte-SAand platelet-rich fibrin (L-PRF) are one of the alternatives to bone autograft to solve this challenge. This study aims to evaluate the effects of allogeneic and xenogeneic lyophilized L-PRF on bone healing in a critical defect of radius bone in rat. Methods: A defect with a diameter of 5 mm was created in the radius bone of 60 rats in four groups. The defect was left empty in the untreated group, and it was filled with autogenous bone graft, allogeneic, and xenogeneic lyophilized L-PRF, respectively, in the other three groups. Radiographic evaluation was done every two weeks, and histopathological evaluation in the 14th, 28th, and 56th days after surgery. Results: The radiographic scores of allogeneic and xenogeneic lyophilized L-PRF groups were significantly higher than the untreated group in all times (P < 0.05). In connection with histopathological Emery's scoring system, the score of allogeneic lyophilized L-PRF was significantly higher than the untreated group (P < 0.05) in the 14th and 28th days after surgery. The score of the xenogeneic lyophilized L-PRF group was also higher than the untreated group, but the difference was not significant (P > 0.05). The allogeneic and xenogeneic lyophilized LPRF scores were significantly higher than the untreated group (P < 0.05) on the 56th day. Conclusion: The results of the present study showed that the allogeneic and xenogeneic lyophilized L-PRF can improve bone healing in the critical radius bone defect in rat model of study.