Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors

被引:3
|
作者
Osaki, Ayumu [1 ]
Kagami, Kazuki [1 ]
Ishinoda, Yuki [1 ]
Sato, Atsushi [1 ]
Kimura, Toyokazu [1 ]
Horii, Shunpei [1 ]
Ito, Kei [1 ]
Toya, Takumi [1 ]
Ido, Yasuo [1 ]
Namba, Takayuki [1 ]
Masaki, Nobuyuki [1 ]
Nagatomo, Yuji [1 ]
Adachi, Takeshi [1 ]
机构
[1] Natl Def Med Coll, Dept Internal Med 1, Div Cardiovasc Med, 3-2 Namiki, Tokorozawa 3598513, Japan
关键词
type 2 diabetes mellitus; steatohepatitis; hypercholesterolemia; perivascular adipose tissue; endothelial dysfunction; oxidative stress; NITRIC-OXIDE; LIVER-DISEASE; OXIDATIVE STRESS; INSULIN; HYPERCHOLESTEROLEMIA; RESISTANCE; DEFICIENT; FIBROSIS; SENSITIVITY; APOPTOSIS;
D O I
10.3390/ijms24076486
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Metabolic syndrome (Mets) is the major contributor to the onset of metabolic complications, such as hypertension, type 2 diabetes mellitus (DM), dyslipidemia, and non-alcoholic fatty liver disease, resulting in cardiovascular diseases. C57BL/6 mice on a high-fat and high-sucrose diet (HFHSD) are a well-established model of Mets but have minor endothelial dysfunction in isolated aortas without perivascular adipose tissue (PVAT). The purpose of this study was to evaluate the effects of additional factors such as DM, dyslipidemia, and steatohepatitis on endothelial dysfunction in aortas without PVAT. Here, we employed eight-week-old male C57BL/6 mice fed with a normal diet (ND), HFHSD, steatohepatitis choline-deficient HFHSD (HFHSD-SH), and HFHSD containing 1% cholesterol and 0.1% deoxycholic acid (HFHSD-Chol) for 16 weeks. At week 20, some HFHSD-fed mice were treated with streptozocin to develop diabetes (HFHSD-DM). In PVAT-free aortas, the endothelial-dependent relaxation (EDR) did not differ between ND and HFHSD (p = 0.25), but in aortas with PVAT, the EDR of HFHSD-fed mice was impaired compared with ND-fed mice (p = 0.005). HFHSD-DM, HFHSD-SH, and HFHSD-Chol impaired the EDR in aortas without PVAT (p < 0.001, p = 0.019, and p = 0.009 vs. ND, respectively). Furthermore, tempol rescued the EDR in those models. In the Mets model, the EDR is compromised by PVAT, but with the addition of DM, dyslipidemia, and SH, the vessels themselves may result in impaired EDR.
引用
收藏
页数:15
相关论文
共 50 条
  • [21] Spirulina Lipids Alleviate Oxidative Stress and Inflammation in Mice Fed a High-Fat and High-Sucrose Diet
    Yang, Yuhong
    Du, Lei
    Hosokawa, Masashi
    Miyashita, Kazuo
    MARINE DRUGS, 2020, 18 (03)
  • [22] Sulforaphane improves leptin responsiveness in high-fat high-sucrose diet-fed obese mice
    Shawky, Noha M.
    Segar, Lakshman
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2018, 835 : 108 - 114
  • [23] Dysfunction of perivascular adipose tissue in mesenteric artery is restored by aerobic exercise in high-fat diet induced obesity
    Liao, Jingwen
    Yin, Honggang
    Huang, Junhao
    Hu, Min
    CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2021, 48 (05) : 697 - 703
  • [24] Anti-contractile effects of perivascular adipose tissue in thoracic aorta from rats fed a high-fat diet: role of aerobic exercise training
    Araujo, Hygor N.
    Victorio, Jamaira A.
    Valgas da Silva, Carmem P.
    Sponton, Amanda C. S.
    Vettorazzi, Jean F.
    de Moraes, Camila
    Davel, Ana P.
    Zanesco, Angelina
    Delbin, Maria A.
    CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2018, 45 (03) : 293 - 302
  • [25] Endothelial Dysfunction and Inflammation Precedes Elevations in Blood Pressure Induced by a High-Fat Diet
    Oishi, Jorge Camargo
    Castro, Cynthia Aparecida
    Silva, Karina Ana
    Fabricio, Victor
    Carnio, Evelin Capelari
    Philips, Shane A.
    Garcia de Oliveira Duarte, Ana Claudia
    Rodrigues, Gerson Jhonatan
    ARQUIVOS BRASILEIROS DE CARDIOLOGIA, 2018, 110 (06) : 558 - 567
  • [26] Hepatic metabolic adaptation and adipose tissue expansion are altered in mice with steatohepatitis induced by high-fat high sucrose diet
    Baiges-Gaya, Gerard
    Fernandez-Arroyo, Salvador
    Luciano-Mateo, Fedra
    Cabre, Noemi
    Rodriguez-Tomas, Elisabet
    Hernandez-Aguilera, Anna
    Castane, Helena
    Romeu, Marta
    Nogues, Maria-Rosa
    Camps, Jordi
    Joven, Jorge
    JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2021, 89
  • [27] Effects of High-Fat and High-Fat/High-Sucrose Diet-Induced Obesity on PVAT Modulation of Vascular Function in Male and Female Mice
    Victorio, Jamaira A.
    Guizoni, Daniele M.
    Freitas, Israelle N.
    Araujo, Thiago R.
    Davel, Ana P.
    FRONTIERS IN PHARMACOLOGY, 2021, 12
  • [28] High-fat Diet Reduces Beneficial Effect Of Perivascular Adipose Tissue On Arterial Stiffness
    McClintock, Dillon
    Bush, Sydney
    Flood, Emma D.
    Watts, Stephanie W.
    Tykocki, Nathan R.
    Roccabianca, Sara
    HYPERTENSION, 2023, 80
  • [29] Effects of tempol on endothelial and vascular dysfunctions and insulin resistance induced by a high-fat high-sucrose diet in the rat
    Bourgoin, Frederic
    Bachelard, Helene
    Badeau, Mylene
    Lariviere, Richard
    Nadeau, Andre
    Pitre, Maryse
    CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2013, 91 (07) : 547 - 561
  • [30] Perivascular adipose tissue reduces serotonin contraction in aorta from rats fed with high fat diet: Effects of exercise training
    Delbin, Maria A.
    Valgas da Silva, Carmem P.
    Victorio, Jamaira A.
    Araujo, Hygor N.
    Davel, Ana Paula C.
    de Moares, Camila
    Antunes, Edson
    Zanesco, Angelina
    ANGIOGENESIS, 2014, 17 (01) : 320 - 321