Epoxide Hydrolases: Multipotential Biocatalysts

被引：22

作者：

Bucko, Marek ^{[1
]}

Kaniakova, Katarina ^{[2
]}

Hronska, Helena ^{[2
]}

Gemeiner, Peter ^{[1
]}

Rosenberg, Michal ^{[2
]}

机构：

[1] Slovak Acad Sci, Inst Chem, Ctr Glycom, Dept Glycobiotechnol, Dubravska Cesta 9, Bratislava 84538, Slovakia

[2] Slovak Univ Technol Bratislava, Inst Biotechnol, Fac Chem & Food Technol, Radlinskeho 9, Bratislava 81237, Slovakia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 08期

关键词：

epoxide hydrolase; epoxides; chiral precursors; biocatalysis; enantioselectivity; enzyme engineering; stabilization; immobilization; enzyme cascade; AGROBACTERIUM-RADIOBACTER AD1; HYDROLYTIC KINETIC RESOLUTION; CIS-EPOXYSUCCINATE HYDROLASE; RACEMIC STYRENE OXIDE; DIRECTED EVOLUTION; MICROBIOLOGICAL TRANSFORMATIONS; CASCADE BIOCATALYSIS; ASPERGILLUS-NIGER; ENZYMATIC TRANSFORMATIONS; ASYMMETRIC HYDROLYSIS;

D O I：

10.3390/ijms24087334

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epoxide hydrolases are attractive and industrially important biocatalysts. They can catalyze the enantioselective hydrolysis of epoxides to the corresponding diols as chiral building blocks for bioactive compounds and drugs. In this review article, we discuss the state of the art and development potential of epoxide hydrolases as biocatalysts based on the most recent approaches and techniques. The review covers new approaches to discover epoxide hydrolases using genome mining and enzyme metagenomics, as well as improving enzyme activity, enantioselectivity, enantioconvergence, and thermostability by directed evolution and a rational design. Further improvements in operational and storage stabilization, reusability, pH stabilization, and thermal stabilization by immobilization techniques are discussed in this study. New possibilities for expanding the synthetic capabilities of epoxide hydrolases by their involvement in non-natural enzyme cascade reactions are described.

引用

页数：27

共 164 条

[71] Preparing β-blocker (R)-Nifenalol based on enantioconvergent synthesis of (R)-p-nitrophenylglycols in continuous packed bed reactor with epoxide hydrolase [J].