Phytochemical profiling, antibacterial, and antibiofilm activities of Sargassum sp. (brown algae) from the Red Sea: ADMET prediction and molecular docking analysis

被引：16

作者：

Alreshidi, Mousa ^{[1
,2
]}

Badraoui, Riadh ^{[1
,3
,4
]}

Adnan, Mohd ^{[1
]}

Patel, Mitesh ^{[5
,6
]}

Alotaibi, Ahmed ^{[7
]}

Saeed, Mohd ^{[1
]}

Ghandourah, Mohammed ^{[8
]}

Al-Motair, Khalid A. ^{[2
]}

Arif, Ibrahim A. ^{[9
]}

Albulaihed, Yazeed ^{[2
]}

Snoussi, Mejdi ^{[1
,10
]}

机构：

[1] Univ Hail, Coll Sci, Dept Biol, POB 2440, Hail, Saudi Arabia

[2] Univ Hail, Mol Diagnost & Personalized Therapeut Unit, Hail, Saudi Arabia

[3] Univ Tunis El Manar, Med Fac Tunis, Sect Histol Cytol, La Rabta Tunis 1017, Tunisia

[4] Univ Sfax, Med Fac Sfax, Lab Histoembryol & Cytogenet, Sfax 3029, Tunisia

[5] Parul Univ, Parul Inst Appl Sci, Dept Biotechnol, Vadodara 391760, Gujarat, India

[6] Parul Univ, Ctr Res Dev, Vadodara 391760, Gujarat, India

[7] Univ Hail, Coll Sci, Dept Chem, POB 2440, Hail 81441, Saudi Arabia

[8] King Abdulaziz Univ, Fac Marine Sci, Dept Marine Chem, POB 80207, Jeddah 21589, Saudi Arabia

[9] King Saud Univ, Coll Sci, Bot & Microbiol Dept, Riyadh, Saudi Arabia

[10] Univ Monastir, High Inst Biotechnol, Lab Genet Biodivers & Valorisat Bioressources, Monastir 5000, Tunisia

来源：

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2023年 / 69卷

关键词：

Sargassum sp; Phytochemical composition; Antimicrobial; Antibiofilm; ADME; Molecular docking; ANTIOXIDANT PROPERTIES; IN-VITRO; STAPHYLOCOCCUS-AUREUS; SEAWEEDS; EXTRACTS;

D O I：

10.1016/j.algal.2022.102912

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Brown algae from the Red sea are a rich source of bioactive molecules with high biological activities. The present work was aimed at studying the phytochemical composition of the methanol/water extract from Sargassum sp. collected from Al-Kharrar Lagoon (Red sea, Saudi Arabia). The antimicrobial and antibiofilm activities were tested against S. aureus (methicillin resistant/sensitive strains), E. coli, K. pneumoniae, S. epidermidis, and C. albicans strains. The obtained results showed that 2-pentadecanone, 6,10,14-trimethyl-, hexadecenoic acid, methyl ester, n-hexadecanoic acid, 1,2-benzenedicarboxylic acid, and mono(2-ethylhexyl) ester were the dominant compound in the methanol/water extract from Sargassum spp. extract. In addition, the same crude extract was effective against all tested microorganisms with a diameter of growth inhibition zone ranging from (10.67 +/- 0.58 mm) for methicillin sensitive-S. aureus to (44.67 +/- 1.15 mm) for S. epidermidis. Minimal inhibitory concentrations and minimal bactericidal concentrations ranged from 6.25-12.5 mg/L to 50->50 mg/mL, respectively. Interestingly, the tested methanol/water extract was able to inhibit the biofilm formation by Staphylococcus spp. strains in a concentration dependent manner, and the highest percentage was recorded at 12.5 mg/mL for S. epidermidis (82.35 +/- 0.03 %). These findings have been confirmed by in silico results, specifically the drug-ability, free binding energies and molecular interactions of the extract compounds with some targeted receptors. Potential antimicrobial and antibiofilm activities could be attributed to the Sargassum sp. for which the phytochemical compounds possessed promising drug-likeness and pharmacokinetic properties.

引用

页数：12