The protection effect of rhodionin against methicillin-resistant Staphylococcus aureus-induced pneumonia through sortase A inhibition

被引：15

作者：

Wang, Xingye ^{[1
,2
]}

Luan, Yanhe ^{[3
]}

Hou, Juan ^{[1
]}

Jiang, Tao ^{[1
]}

Zhao, Yicheng ^{[1
]}

Song, Wu ^{[1
]}

Wang, Li ^{[1
]}

Kong, Xiangri ^{[1
,3
]}

Guan, Jiyu ^{[4
]}

Song, Danning ^{[1
]}

Wang, Bingmei ^{[1
]}

Li, Mingquan ^{[1
,2
,3
]}

机构：

[1] Changchun Univ Chinese Med, Changchun, Peoples R China

[2] Changchun Univ Chinese Med, Affiliated Hosp 3, Changchun, Peoples R China

[3] Changchun Univ Chinese Med, Affiliated Hosp, Changchun, Peoples R China

[4] Jilin Univ, Coll Vet Med, Key Lab Zoonosis, Minist Educ, Changchun, Peoples R China

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2023年 / 39卷 / 01期

关键词：

Anti-virulence; MRSA; Rhodionin; Sortase A; SURFACE-PROTEINS; CELL-WALL; PHENOLIC-COMPOUNDS; BACTERIAL; FLAVONOIDS; EMERGENCE; SUBSTRATE; UPDATE; ROOT;

D O I：

10.1007/s11274-022-03457-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Methicillin-resistant Staphylococcus aureus (MRSA) is a zoonotic antibiotic-resistant pathogen that negatively impacts society from medical, veterinary, and societal standpoints. The search for alternative therapeutic strategies and innovative anti-infective agents is urgently needed. Among the pathogenic mechanisms of Staphylococcus aureus (S. aureus), sortase A is a virulence factor of great concern because it is highly linked with the ability of MRSA to invade the host. In this study, we identified that rhodionin, a natural compound of flavonoid glucosides, effectively inhibited the activity of SrtA without affecting the survival and growth of bacteria, and its half maximal inhibitory concentration (IC50) value was 22.85 mu g/mL. In vitro, rhodionin prominently attenuated the virulence-related phenotype of SrtA by reducing the adhesion of S. aureus to fibrinogen, reducing the capacity of protein A (SpA) on the bacterial surface and biofilm formation. Subsequently, fluorescence quenching and molecular docking were performed to verify that rhodionin directly bonded to SrtA molecule with K-A value of 6.22 x10(5) L/mol. More importantly, rhodionin showed a significant protective effect on mice pneumonia model and improved the survival rate of mice. According to the above findings, rhodionin achieved efficacy in the treatment of MRSA-induced infections, which holds promising potential to be developed into a candidate used for MRSA-related infections. [GRAPHICS] .

引用

页数：14