Compact fiber sensor for rapid trypsin detection based on the whispering gallery mode with a liquid crystal microdroplet

被引:2
作者
Ma, Zhenyu [1 ,2 ]
Sun, Jing [1 ,2 ]
Li, Xiaolan [1 ,2 ]
Shan, Wenjun [3 ]
Yan, Yunxiang [1 ,2 ,4 ]
Liu, Yongjun [1 ,2 ]
机构
[1] Harbin Engn Univ, Key Lab Infiber Integrated Opt, Minist Educ, Harbin 150001, Peoples R China
[2] Harbin Engn Univ, Key Lab Photon Mat & Devices Phys Ocean Applicat, Minist Ind & Informat Technol, Harbin 150001, Peoples R China
[3] Guangzhou Mech Engn Res Inst Co Ltd, Guangzhou 510700, Peoples R China
[4] Harbin Engn Univ, Qingdao Innovat & Dev Ctr, Qingdao 266000, Peoples R China
基金
中国国家自然科学基金;
关键词
Trypsin detection; Liquid crystal microdroplet; Whispering gallery mode; Low sample consumption; RESONANCE ENERGY-TRANSFER; GOLD NANOCLUSTERS; SURFACTANT; PH; QUANTIFICATION; NANOPARTICLES; ASSAY;
D O I
10.1016/j.snb.2023.135125
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Trypsin, a protease produced in the pancreas, plays a crucial role in various medical conditions, including acute pancreatitis, cystic fibrosis, and malnutrition, where abnormal trypsin levels are often implicated. Consequently, trypsin detection has emerged as a valuable diagnostic tool in clinical medicine. In this study, we introduced an integrated fiber sensor that capitalizes on the amplification and transduction capabilities of liquid crystals, coupled with the high sensitivity of the whispering gallery mode, to achieve precise trypsin detection. This innovative approach offers consecutive measurement and rapid response times, typically under 200 s, and remarkably low sample consumption, as little as 3 nL, potential for detection in capillary blood sampling. Furthermore, when subjected to validation tests for trypsin detection in human serum, our method demonstrated excellent consistency and reliability, with recovery ratios falling within the range of 97-112%. Beyond trypsin, this method's adaptability and versatility make it a universal technique with potential applications in the field of biosensing and biochemistry.
引用
收藏
页数:9
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