Effect of Europinidin against Alcohol-Induced Liver Damage in Rats by Inhibiting the TNF-α/TGF-β/IFN-γ/NF-kB/Caspase-3 Signaling Pathway

Background: The effect of europinidin on alcoholic liverdamagein rats was examined in this research. Methods: A total of 24 Wistarrats were grouped in the same way into four groups: normal control(normal), ethanol control (EtOH), europinidin low dose (10 mg/kg),and europinidin higher dose (20 mg/kg). The test group rats were orallytreated with europinidin-10 and europinidin-20 for 4 weeks, whereas5 mL/kg distilled water was administered to control rats. In addition,1 h after the last dose of the above-mentioned oral treatment, 5 mL/kg(i.p.) EtOH was injected to induce liver injury. After 5 h of EtOHtreatment, samples of blood were withdrawn for biochemical estimations.Results: Administration of europinidin at both doses restored allof the estimated serum, i.e., liver function tests (ALT, AST, ALP),biochemical test (Creatinine, albumin, BUN, direct bilirubin, andLDH), lipid assessment (TC and TG), endogenous antioxidants (GSH-Px,SOD, and CAT), malondialdehyde (MDA), nitric oxide (NO), cytokines(TGF-beta, TNF-alpha, IL-1 beta, IL-6, IFN-gamma, and IL-12),caspase-3, and nuclear factor kappa B (NF-kappa B) associated withthe EtOH group. Conclusion: The results of the investigation showedthat europinidin had favorable effects in rats given EtOH and mayhave hepatoprotective potential property.