Prefabricated platinum nanomaterial matrix for MALDI-MS imaging of oligosaccharides and lipids in plant tissues

被引:6
|
作者
Shen, Yu-Lin [1 ]
Zhuang, Si-Jia [1 ]
Yang, Fan [1 ]
Gong, Can [1 ]
Xu, Xu [1 ]
机构
[1] Shanghai Inst Technol, Sch Chem & Environm Engn, Shanghai, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2023年 / 14卷
关键词
matrix-assisted laser desorption ionization mass spectrometry imaging; matrix; platinum nanomaterials; plant tissue; oligosaccharide; lipid; MASS-SPECTROMETRY;
D O I
10.3389/fpls.2023.1105374
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) can visualize the spatial distribution characteristics of molecules in tissues in situ, in which the matrix plays a key role. In this paper, we propose a platinum nanomaterial pre-coated matrix, which can be prepared in bulk by sputtering platinum nanoparticles onto slides using an ion sputterer and then used for MALDI-MS analysis by placing tissue sections on the matrix. We used this matrix for MALDI-MS imaging analysis of corn kernels and germinated wheat sections, and the results show that triacylglycerides were mainly distributed in the embryo of corn kernels and germinated wheat, and sugars were mainly distributed in the endosperm, with the highest content of disaccharides.It provides a simple and reliable experimental condition for analyzing the distribution of oligosaccharide and lipid components in plant tissues.
引用
收藏
页数:5
相关论文
共 50 条
  • [1] Matrix Recrystallization for MALDI-MS Imaging of Maize Lipids at High-Spatial Resolution
    Duenas, Maria Emilia
    Carlucci, Laura
    Lee, Young Jin
    JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2016, 27 (09) : 1575 - 1578
  • [2] Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles
    Shelley N. Jackson
    Kathrine Baldwin
    Ludovic Muller
    Virginia M. Womack
    J. Albert Schultz
    Carey Balaban
    Amina S. Woods
    Analytical and Bioanalytical Chemistry, 2014, 406 : 1377 - 1386
  • [3] Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles
    Jackson, Shelley N.
    Baldwin, Kathrine
    Muller, Ludovic
    Womack, Virginia M.
    Schultz, J. Albert
    Balaban, Carey
    Woods, Amina S.
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 406 (05) : 1377 - 1386
  • [4] MALDI-MS imaging of lipids in ex vivo human skin
    Philippa J. Hart
    Simona Francese
    Emmanuelle Claude
    M. Nicola Woodroofe
    Malcolm R Clench
    Analytical and Bioanalytical Chemistry, 2011, 401 : 115 - 125
  • [5] MALDI-MS imaging of lipids in ex vivo human skin
    Hart, Philippa J.
    Francese, Simona
    Claude, Emmanuelle
    Woodroofe, M. Nicola
    Clench, Malcolm R.
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 401 (01) : 115 - 125
  • [6] Highly Multiplexed Immunohistochemical MALDI-MS Imaging of Biomarkers in Tissues
    Yagnik, Gargey
    Liu, Ziying
    Rothschild, Kenneth J.
    Lim, Mark J.
    JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2021, 32 (04) : 977 - 988
  • [7] MALDI-MS imaging of lipids in corn using a flexible ultrasonic spraying device as matrix deposition method
    Pei, Xing-Li
    Liu, Xiao-Ning
    Du, Jia-Lin
    Gong, Can
    Xu, Xu
    INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2020, 455
  • [8] A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids
    Leopold, Jenny
    Prabutzki, Patricia
    Engel, Kathrin M.
    Schiller, Juergen
    BIOMOLECULES, 2023, 13 (03)
  • [9] Enhanced in situ detection and imaging of lipids in biological tissues by using 2,3-dicyanohydroquinone as a novel matrix for positive-ion MALDI-MS imaging
    Liu, Yaqin
    Chen, Lulu
    Qin, Liang
    Han, Manman
    Li, Jinming
    Luo, Feixian
    Xue, Kun
    Feng, Jinchao
    Zhou, Yijun
    Wang, Xiaodong
    CHEMICAL COMMUNICATIONS, 2019, 55 (83) : 12559 - 12562
  • [10] Hydrazinonicotinic acid as a novel matrix for highly sensitive and selective MALDI-MS analysis of oligosaccharides
    Jiao, Jing
    Zhang, Ying
    Yang, Pengyuan
    Lu, Haojie
    ANALYST, 2015, 140 (01) : 156 - 161