A sensitive, robust and high-throughput isotope dilution LC-MS/MS method for quantifying three folate forms in serum

被引：5

作者：

Cao, Yunfeng ^{[1
]}

Zeng, Jia ^{[1
]}

Huang, Ting ^{[1
]}

Chen, Jianxing ^{[1
]}

机构：

[1] Shanghai Inst Biomed & Pharmaceut Technol, NHC Key Lab Reprod Regulat, Dev Platform Mass Spectrometry Technol, Laohumin Rd 779, Shanghai 200232, Peoples R China

来源：

BIOANALYSIS | 2023年 / 15卷 / 05期

基金：

中国国家自然科学基金;

关键词：

5-formyltetrahydrofolate; 5-methyltetrahydrofolate; 96-well solid phase extraction; folic acid; ID-LC-MS; MS; sensitivity; throughput; TANDEM MASS-SPECTROMETRY; FOLIC-ACID; QUANTITATIVE-DETERMINATION; VITAMERS; 5-METHYLTETRAHYDROFOLATE; HOMOCYSTEINE; METHIONINE; HEALTH;

D O I：

10.4155/bio-2023-0007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aimed to establish an isotope dilution LC-MS/MS method for the determination of folic acid, 5-formyltetrahydrofolate and 5-methyltetrahydrofolate in human serum. This method was then used to quantify these three folate forms in the healthy adult population and supplement users. A stable 96-well solid-phase extraction system was used to prepare serum samples. The highly sensitive method was established using a Shimadzu LCMS-8060NX. The linearity was good in the range of 0.1-10 nmol/l for folic acid and 5-formyltetrahydrofolate and 1.0-100 nmol/l for 5-methyltetrahydrofolate. The accuracy and precision were good. The method was sensitive, robust and high-throughput and could be used for the routine clinical monitoring of these three folate forms in the Chinese population.

引用

页码：249 / 258

页数：10

共 24 条

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