Multiplex PCR for detection of acquired plasmid-borne fosfomycin resistance fos genes in Escherichia coli

被引：3
作者：
Freire, Samanta [1 ]
Grilo, Teresa [1 ]
Nordmann, Patrice [2 ,3 ,4 ]
Poirel, Laurent [2 ,3 ,4 ]
Aires-de-Sousa, Marta [1 ,5 ,6 ]
机构：
[1] Portuguese Red Cross, Lab Mol Biol, Lisbon, Portugal
[2] Univ Fribourg, Fac Sci & Med, Med & Mol Microbiol Unit, Fribourg, Switzerland
[3] Univ Fribourg, INSERM European Unit, France, Fribourg, Switzerland
[4] Swiss Natl Reference Ctr Emerging Antibiot Resista, Fribourg, Switzerland
[5] Escola Super Saude Cruz Vermelha Portuguesa Lisboa, Lisbon, Portugal
[6] Univ Nova Lisboa UNL, Lab Mol Genet, Inst Tecnol Quim & Biol Antonio Xavier ITQB, Oeiras, Portugal
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2023年 / 105卷 / 02期
基金：
瑞士国家科学基金会;
关键词：
fosfomycin resistance; fosA; fosC; fosL; Escherichia coli; multiplex PCR; KLEBSIELLA-PNEUMONIAE ORIGIN; IDENTIFICATION;
D O I：
10.1016/j.diagmicrobio.2022.115864
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A rapid (<3 hours) and reliable multiplex PCR was developed for detecting simultaneously known plasmid-mediated fos genes conferring acquired resistance to fosfomycin. Our technique was tested on a collection of Escherichia coli isolates previously identified as bearing the fosA-, fosC- and fosL-like genes, showing a sensi-tivity and a specificity of 100%.(c) 2022 Elsevier Inc. All rights reserved.
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页数：3
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