Multiplex PCR for detection of acquired plasmid-borne fosfomycin resistance fos genes in Escherichia coli

被引：3

作者：

Freire, Samanta ^{[1
]}

Grilo, Teresa ^{[1
]}

Nordmann, Patrice ^{[2
,3
,4
]}

Poirel, Laurent ^{[2
,3
,4
]}

Aires-de-Sousa, Marta ^{[1
,5
,6
]}

机构：

[1] Portuguese Red Cross, Lab Mol Biol, Lisbon, Portugal

[2] Univ Fribourg, Fac Sci & Med, Med & Mol Microbiol Unit, Fribourg, Switzerland

[3] Univ Fribourg, INSERM European Unit, France, Fribourg, Switzerland

[4] Swiss Natl Reference Ctr Emerging Antibiot Resista, Fribourg, Switzerland

[5] Escola Super Saude Cruz Vermelha Portuguesa Lisboa, Lisbon, Portugal

[6] Univ Nova Lisboa UNL, Lab Mol Genet, Inst Tecnol Quim & Biol Antonio Xavier ITQB, Oeiras, Portugal

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2023年 / 105卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

fosfomycin resistance; fosA; fosC; fosL; Escherichia coli; multiplex PCR; KLEBSIELLA-PNEUMONIAE ORIGIN; IDENTIFICATION;

D O I：

10.1016/j.diagmicrobio.2022.115864

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A rapid (<3 hours) and reliable multiplex PCR was developed for detecting simultaneously known plasmid-mediated fos genes conferring acquired resistance to fosfomycin. Our technique was tested on a collection of Escherichia coli isolates previously identified as bearing the fosA-, fosC- and fosL-like genes, showing a sensi-tivity and a specificity of 100%.(c) 2022 Elsevier Inc. All rights reserved.

引用

页数：3

共 23 条

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