Cell sorters see things more clearly now

被引：9

作者：

Schraivogel, Daniel ^{[1
]}

Steinmetz, Lars M. ^{[1
,2
,3
]}

机构：

[1] Genome Biol Unit, European Mol Biol Lab EMBL, Heidelberg, Germany

[2] Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA

[3] Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA

来源：

MOLECULAR SYSTEMS BIOLOGY | 2023年 / 19卷 / 03期

基金：

欧洲研究理事会;

关键词：

D O I：

10.15252/msb.202211254

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microscopy and fluorescence-activated cell sorting (FACS) are two of the most important tools for single-cell phenotyping in basic and biomedical research. Microscopy provides high-resolution snapshots of cell morphology and the inner workings of cells, while FACS isolates thousands of cells per second using simple parameters, such as the intensity of fluorescent protein labels. Recent technologies are now combining both methods to enable the fast isolation of cells with microscopic phenotypes of interest, thereby bridging a long-standing gap in the life sciences. In this Commentary, we discuss the technical advancements made by image-enabled cell sorting and highlight novel experimental strategies in functional genomics and single-cell research.

引用

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