Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua

被引:5
|
作者
Wu, Han [1 ,2 ]
Zhao, Dan [1 ]
Guo, Xiao-Chang [1 ]
Liu, Zhao-Rui [1 ]
Li, Rui-Jun [1 ]
Lu, Xiu-Jun [1 ]
Guo, Wei [1 ,2 ]
机构
[1] Hebei Agr Univ, Coll Plant Protect, Baoding 071001, Peoples R China
[2] Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China
基金
中国国家自然科学基金;
关键词
chitin deacetylase; Spodoptera exigua; RNA interference; midgut; peritrophic membrane; PERITROPHIC MEMBRANE; BOMBYX-MORI; PHYLOGENETIC ANALYSIS; DOMAIN ORGANIZATION; IDENTIFICATION; MATRIX; LEPIDOPTERA; EXPRESSION; SILKWORM; PROTEINS;
D O I
10.3390/ijms24043076
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects. Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae. The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively. The deduced protein sequences showed that SeCDAs are synthesized as preproteins of 387, 378, 385 and 383 amino acid residues, respectively. It was revealed via spatiotemporal expression analysis that SeCDAs were more abundant in the anterior region of the midgut. The SeCDAs were down-regulated after treatment with 20-hydroxyecdysone (20E). After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated. After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed. The vesicles in the midgut were small and more fragmented or disappeared after SeCDAs were silenced. Additionally, the PM structure was scarce, and the chitin microfilament structure was loose and chaotic. It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua. Additionally, the midgut tissue and the PM structure and composition were affected by Group V CDAs.
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页数:15
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