Hybrid Fe3O4-Au Nanoparticles Prepared by Two-Step Pulsed Laser Ablation in Liquid for Biomedical Applications

The nanosecond Nd:YAG pulsed laser ablation in liquid (PLAL) technique was used to successfully synthesize hybrid Fe3O4-Au nanoparticles (NPs). The synthesized nanoparticles were investigated using different techniques such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), UV-VIS spectroscopy, field emission scanning electron microscopy (FESEM) with energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), UV-VIS spectroscopy, and photoluminescent spectroscopy (PL). Then the NP concentration in the bacterial pathogen was investigated. The FTIR and XRD data confirmed the successful synthesis of Fe3O4 NPs doped with Au. The morphology of hybrid Fe3O4-Au NPs was observed to be agglomerated into semispherical nanostructure particles. The absorption and PL properties of the nanoparticles were found to vary with doping concentrations. Additionally, bacterial activity was effectively inhibited by the hybrid NPs, which were found to be more effective against gram-negative and gram-positive strains, with a highly inhibited zone against Acinetobacter baumannii. Also, the inhibition zone increased with increasing doping concentrations of NPs.