Evaluation of the Aptima BV and CV/TV assays compared to conventional laboratory based testing methods for the diagnosis of vaginitis

被引：4

作者：

Caza, Melissa ^{[1
,2
]}

Charles, Marthe ^{[2
,3
]}

Locher, Kerstin ^{[2
,3
]}

Hoang, Linda ^{[2
,4
]}

Tucker, Morgan ^{[5
]}

Mandy, Jeremy ^{[5
]}

Jewsbury, Heather ^{[1
]}

Wilmer, Amanda ^{[1
,2
]}

机构：

[1] Kelowna Gen Hosp, Kelowna, BC, Canada

[2] Univ British Columbia, Vancouver, BC, Canada

[3] Vancouver Gen Hosp, Vancouver, BC, Canada

[4] BCCDC Publ Hlth Microbiol & Reference Lab, Vancouver, BC, Canada

[5] Univ British Columbia Okanagan, Kelowna, BC, Canada

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2023年 / 106卷 / 04期

关键词：

Vaginitis; Women's health Molecular assay; STI; TRICHOMONAS-VAGINALIS;

D O I：

10.1016/j.diagmicrobio.2023.115953

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Purpose: Vaginitis is caused by bacterial vaginosis (BV), Candida vaginitis (CV) and Trichomonas vaginalis (TV). This retrospective study evaluates the performance of the Aptima CV/TV, and BV assays on the automated Panther system.Methods: Two hundred forty-two multitest swabs were tested on the CV/TV assay and 422 on the BV assay. Positive and negative percent agreement (PPA, NPA) of the Candida glabrata (CG), Candida species group (CSG), TV and BV targets were calculated using a modified gold standard, with review of Gram smear and the usage of the Allplex Vaginitis Screening Assay to resolve discrepancies.Results: The PPA and NPA were respectively 98.4% and 95.9% for BV, 100% and 95.4% for CSG, 100% and 99% for CG, and 100% and 100% for TV, and when compared to consensus results.Conclusion: The CV/TV and BV assays surpassed the acceptance criteria threshold of 95%, and proved to be an excellent alternative to conventional testing. & COPY; 2023 Elsevier Inc. All rights reserved.

引用

页数：6

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