Image-Based Gel Encapsulation of Suspended Single Cells for Parallel Single-Cell Screening

被引：2

作者：

Selvam, Venkatesh Kumar Panneer ^{[1
]}

Bin Kamaludin, Muhammad Luqman Arief ^{[1
]}

Murtaza, Ghulam ^{[1
]}

Chowdhury, Rifat Hussain ^{[1
]}

Debnath, Tanmay ^{[1
]}

Okamoto, Shunya ^{[1
]}

Shibata, Takayuki ^{[1
]}

Santra, Tuhin Subhra ^{[2
]}

Nagai, Moeto ^{[1
,3
]}

机构：

[1] Toyohashi Univ Technol, Dept Mech Engn, 1-1 Hibarigaoka Tempaku Cho, Toyohashi, Aichi 4418580, Japan

[2] Indian Inst Technol, Dept Engn Design, Chennai 600036, Tamil Nadu, India

[3] Toyohashi Univ Technol, Inst Res Next Generat Semicond & Sensing Sci, 1-1 Hibarigaoka, Tempaku Cho, Toyohashi, Aichi 4418580, Japan

来源：

JOURNAL OF ROBOTICS AND MECHATRONICS | 2023年 / 35卷 / 05期

关键词：

multi-light irradiation; photo-crosslinkable gel; cell en-capsulation; single-cell screening; image processing;

D O I：

10.20965/jrm.2023.p1177

中图分类号：

TP24 [机器人技术];

学科分类号：

080202 ; 1405 ;

摘要：

Single-cell screening, which has revolutionized the life sciences, is an important method for detecting, separating, or treating specific cells based on desired characteristics. Previously, single cells of interest were manually identified in an image, which required human labor and time. We developed an automated photopolymerization system to encapsulate suspended single cells in approximately 50-mu m photo-crosslinkable hydrogel squares. An image was captured, and single cells were selected from grouped cells based on image processing. A generated image was transferred to a digital micromirror device (DMD), and in parallel, target-suspended single cells were encapsulated in gelatin methacryloyl (GelMA) hydrogels. We built a data transfer platform based on a Power Automate Desktop (PAD), completed the data transfer, and projected the processed image onto a sample in 10 s, ensuring a minimum alignment error of 6.2 mu m.

引用

页码：1177 / 1184

页数：8

共 31 条

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