3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects

被引:7
作者
Wang, Chenglong [1 ,2 ]
Dong, Jinlei [1 ,2 ]
Liu, Fanxiao [1 ,2 ]
Liu, Nan [1 ,2 ]
Li, Lianxin [1 ,2 ]
机构
[1] Shandong First Med Univ, Shandong Prov Hosp, Dept Orthopaed Surg, Jinan 250021, Peoples R China
[2] Shandong Trauma Ctr, Dept Orthopaed Surg, Jinan 2500021, Peoples R China
关键词
Hydrogel; PCL; Bone defect; SDF-1; alpha; 3D-printed scaffold; Bioactive glass; SUSTAINED-RELEASE; DELIVERY; SDF-1;
D O I
10.1186/s13036-023-00401-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background The long-term nonunion of bone defects is always a difficult problem in orthopaedics treatment. Artificial bone implants made of polymeric materials are expected to solve this problem due to their suitable degradation rate and good biocompatibility. However, the lack of mechanical strength, low osteogenic induction ability and poor hydrophilicity of these synthetic polymeric materials limit their large-scale clinical application.Results In this study, we used bioactive glass (BG) (20%, W/W) and polycaprolactone (PCL, 80%, W/W) as raw materials to prepare a bone repair scaffold (PCL@BG20) using fused deposition modelling (FDM) three-dimensional (3D) printing technology. Subsequently, stromal cell-derived factor-1 alpha (SDF-1 alpha) chemokines were loaded into the PCL@BG20 scaffold pores with gelatine methacryloyl (GelMA) hydrogel. The experimental results showed that the prepared scaffold had a porous biomimetic structure mimicking that of cancellous bone, and the compressive strength (44.89 +/- 3.45 MPa) of the scaffold was similar to that of cancellous bone. Transwell experiments showed that scaffolds loaded with SDF-1 alpha could promote the recruitment of bone marrow stromal cells (BMSCs). In vivo data showed that treatment with scaffolds containing SDF-1 alpha and BG (PCL@BG-GelMA/SDF-1 alpha) had the best effect on bone defect repair compared to the other groups, with a large amount of new bone and mature collagen forming at the bone defect site. No significant organ toxicity or inflammatory reactions were observed in any of the experimental groups.Conclusions The results show that this kind of scaffold containing BG and SDF-1 alpha serves the dual functions of recruiting stem cell migration in vivo and promoting bone repair in situ. We envision that this scaffold may become a new strategy for the clinical treatment of bone defects.
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页数:14
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