CRISPR/Cas12a and primer-assisted rolling circle amplification integrated ultra-sensitive dual-signal sensing platform for EGFR 19 detection

被引：12

作者：

Deng, Liyuan ^{[1
]}

Zhou, Shiying ^{[1
]}

Dong, Jiangbo ^{[1
]}

Liu, Yin ^{[1
]}

Huang, Zhen ^{[4
,5
]}

Sun, Human ^{[1
]}

Jin, Liang ^{[1
]}

Huo, Danqun ^{[1
,2
]}

Hou, Changjun ^{[1
,3
]}

机构：

[1] Chongqing Univ, Bioengn Coll, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants,Minist, Chongqing 400044, Peoples R China

[2] Chongqing Univ, Sch Microelect & Commun Engn, Chongqing Key Lab Biopercept & Intelligent Informa, Chongqing 400044, Peoples R China

[3] Shanghai Jiao Tong Univ, Natl Facil Translat Med, Shanghai 200240, Peoples R China

[4] Sichuan Univ, SeNA Res Inst, Coll Life Sci, Key Lab Bioresource & Ecoenvironm,Minist Educ, Chengdu 610000, Peoples R China

[5] Szostak CDHT Large Nucl Acids Inst, Chengdu 610000, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2023年 / 1279卷

关键词：

EGFR; 19; Rolling circle amplification (RCA); CRISPR/Cas12a; Dual-signal sensor platform; METAL-ORGANIC FRAMEWORKS; ABUNDANCE;

D O I：

10.1016/j.aca.2023.341755

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, we integrated CRISPR/Cas12a with primer-assisted rolling circle amplification (PARCA) to specifically detect EGFR 19 from the genome. We fused the method into fluorescent and electrochemical detection systems forming a stable and sensitive dual-signal sensing platform. The fluorescent detection system stably detected EGFR 19 in a linear range from 500 fM to 10 nM with an ultra-low background signal. The electrochemical detection system possessed a detection limit as low as 42 aM due to the introduction of nanomaterial UIO-66- NH2. The dual-signal sensing platform showed superior performance in complex serum samples and real cell genomes and provided a flexible and dynamic approach for the ultra-sensitive detection of EGFR 19.

引用

页数：8

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[1] A new zirconium inorganic building brick forming metal organic frameworks with exceptional stability [J].