This mixed method study investigated comparatively the effects of instructional video (IV) types used in online learning (OL). A total of 394 pre-service teachers (PTs) participated from two universities in Turkey. Results indicated that the IV type which includes interactive elements and a combination of the human embodiment and instructional contents on screen by using lightboard or green screen technologies are more effective. Not only the details of instructor's position, visibility, narrative style, facial movements, gestures, cues but also real-like learning settings and video duration enhance the effectiveness of IVs. The opinions of PTs were reported under six themes: video length, interaction elements, instructor's gestures and facial expressions, naturality and reality, immersion and flow, dynamic drawing. Implications on IV types, suggestions for future studies were also discussed.