A Strainer-Based Platform for the Collection and Immunolabeling of Porcine Epidemic Diarrhea Virus-Infected Porcine Intestinal Organoid

被引：1

作者：

Liu, Yinju ^{[1
]}

Tan, Jinlong ^{[1
]}

Zhang, Nianzhang ^{[1
]}

Li, Wenhui ^{[1
]}

Fu, Baoquan ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, State Key Lab Anim Dis Control & Prevent, Key Lab Vet Publ Hlth, Agr Minist,Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 21期

关键词：

strainer platform; porcine intestinal organoid; collection; labeling; porcine epidemic diarrhea virus; STEM-CELL; DISEASE; MODELS;

D O I：

10.3390/ijms242115671

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of organoid research has raised new requirements for this methodology. In a previous study, we demonstrated that an emerging protocol achieved the collection, loading, and programmed immunolabeling of mouse intestinal organoids based on a strainer platform. To uncover the applied potential of this novel methodology on organoids from other species, the strainer platform was utilized to characterize the porcine epidemic diarrhea virus (PEDV)-infected porcine intestinal organoid model. Based on a previous study, some steps were changed to improve the efficiency of the assay by simplifying the reagent addition procedure. In addition, we redefined the range of strainer sizes on porcine intestinal organoids, showing that strainers with pore sizes of 40 and 70 mu m matched the above protocol well. Notably, the strainer platform was successfully used to label viral proteins, laying the foundation for its application in the visualization of viral infection models. In summary, the potential of the strainer platform for organoid technology was explored further. More extensive exploration of this platform will contribute to the development of organoid technology.

引用

页数：12

共 43 条

[21] Porcine Intestinal Enteroids: a New Model for Studying Enteric Coronavirus Porcine Epidemic Diarrhea Virus Infection and the Host Innate Response [J].