Opto-microfluidic assisted synthesis of photo-protoporphyrin (pPP) conjugated to hollow gold-albumin hybrid nanoshells to enhance the efficiency of photodynamic therapy of triple negative breast cancer cells

被引：4

作者：

Imanparast, Armin ^{[1
,2
,3
]}

Shaegh, Seyed Ali Mousavi ^{[3
,4
,5
]}

Attaran, Neda ^{[6
]}

Ameri, Amir Reza ^{[3
]}

Sazgarnia, Ameneh ^{[1
,2
]}

机构：

[1] Mashhad Univ Med Sci, Med Phys Res Ctr, Mashhad, Iran

[2] Mashhad Univ Med Sci, Fac Med, Dept Med Phys, Mashhad, Iran

[3] Mashhad Univ Med Sci, BuAli Res Inst, Lab Microfluid & Med Microsyst, Mashhad, Iran

[4] Mashhad Univ Med Sci, Ghaem Hosp, Orthoped Res Ctr, Mashhad, Iran

[5] Mashhad Univ Med Sci, Ghaem Hosp, Clin Res Unit, Mashhad, Iran

[6] Islamic Azad Univ, Appl Biophoton Res Ctr, Dept Med Nanotechnol, Tehran Sci & Res Branch, Tehran, Iran

来源：

PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY | 2023年 / 43卷

基金：

美国国家科学基金会;

关键词：

Microfluidics; Photodynamic therapy; Bovine serum albumin; Photo-protoporphyrin; Hollow gold nanoshells; Triple negative breast cancer; SERUM ALBUMINS; PROTEIN;

D O I：

10.1016/j.pdpdt.2023.103632

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Introduction: Protoporphyrin-IX (PpIX), a photosensitizer used in photodynamic therapy, has limitations due to its hydrophobicity, rapid photobleaching, and low absorption peak in the red region. These limitations make the use of PpIX less effective for photodynamic therapy treatments. In this study, we harnessed the power of microfluidic technology to manipulate the properties of PpIX and quickly synthesize albumin-based hybrid nanoshells with high reproducibility.Methods and material: To begin with, we designed a microfluidic chip with SolidWorks? software; then the chip was fabricated in Poly(methyl methacrylate) (PMMA) material using micromilling and thermal bonding. We synthesized PpIX-loaded CTAB micelles and subsequently transformed the PpIX structure into photo-protoporphyrin (PPP,) by opto-microfluidic chip (Integrating a microfluidic chip with a light source). Simulta-neously with CTAB-PPP synthesis complex, we trapped it in binding sites of bovine serum albumin (BSA). Af-terward, we used the same method (without irradiating) to generate a hybrid nanostructure consisting of hollow gold nanoshells (HGN) and BSACTAB-PPP. Then, after physical characterization of nanostructures, the photo -dynamic effects of the agents (HGNs, CTAB-PpIX, BSA-CTABPpIX, HGN-BSA-CTAB-PpIX, CTAB-PPP, BSA-CTAB-PPP, and HGNs-BSA-CTAB-PPP) were evaluated on MDA-MB-231 and 4T1 cells and the cytotoxic properties of the therapeutic agents after treatment for 24, 48, and 72 hours were investigated using MTT assay. Finally, we analyzed the findings using GraphPad Prism 9.0 software.Results: Results revealed that the opto-microfluidic assisted synthesis of HGN-BSA-CTAB-PPP is highly efficient and reproducible, with a size of 120 nm, a zeta potential of-16 mV, and a PDI index of 0.357. Furthermore, the cell survival analysis demonstrated that the HGNBSA-CTAB-PPP hybrid nanostructure can significantly reduce the survival of MDA-MB-231 and 4T1 cancer cells at low radiation doses (< 10 J/cm2) when exposed to an incoherent light source due to its strong absorption peak at a wavelength of 670 nm.Conclusion: This research indicates that developing albumin-based multidrug hybrid nanostructures using microfluidic technology could be a promising approach to design more efficient photodynamic therapy studies.

引用

页数：14