High expression of RNF169 is associated with poor prognosis in pancreatic adenocarcinoma by regulating tumour immune infiltration

被引：5

作者：

Wang, Jieyan ^{[1
]}

Chen, Hanghang ^{[2
]}

Deng, Qiong ^{[1
]}

Chen, Yeda ^{[1
]}

Wang, Zhu ^{[1
]}

Yan, Zhengzheng ^{[3
]}

Wang, Yinglin ^{[4
]}

Tang, Haoxuan ^{[2
]}

Liang, Hui ^{[1
]}

Jiang, Yong ^{[2
,3
]}

机构：

[1] Southern Med Univ, Peoples Hosp Longhua, Dept Urol, Affiliated Hosp, Shenzhen, Guangdong, Peoples R China

[2] Southern Med Univ, Sch Basic Med Sci, Dept pathophysiol, Guangdong Prov Key Lab Prote,State Key Lab Organ F, Guangzhou, Peoples R China

[3] Southern Med Univ, Affiliated Dongguan Hosp, Dongguan Key Lab Resp & Crit Care Med, Dongguan, Peoples R China

[4] Second Hosp Zhuzhou, Dept Pediat, Zhuzhou, Hunan, Peoples R China

来源：

FRONTIERS IN GENETICS | 2023年 / 13卷

基金：

中国国家自然科学基金;

关键词：

ring finger protein 169; tumour immune infiltration; pancreatic adenocarcinoma; non-coding RNA; bioinformatics; UBIQUITIN-DEPENDENT RESPONSE; CELL-PROLIFERATION; GENE-EXPRESSION; WEB SERVER; CANCER; LIVER;

D O I：

10.3389/fgene.2022.1022626

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: Pancreatic adenocarcinoma (PAAD) is a highly deadly and aggressive tumour with a poor prognosis. However, the prognostic value of RNF169 and its related mechanisms in PAAD have not been elucidated. In this study, we aimed to explore prognosis-related genes, especially RNF169 in PAAD and to identify novel potential prognostic predictors of PAAD. Methods: The GEPIA and UALCAN databases were used to investigate the expression and prognostic value of RNF169 in PAAD. The correlation between RNF169 expression and immune infiltration was determined by using TIMER and TISIDB. Correlation analysis with starBase was performed to identify a potential regulatory axis of lncRNA-miRNA-RNF169. Results: The data showed that the level of RNF169 mRNA expression in PAAD tissues was higher than that in normal tissues. High RNF169 expression was correlated with poor prognosis in PAAD. In addition, analysis with the TISIDB and TIMER databases revealed that RNF169 expression was positively correlated with tumour immune infiltration in PAAD. Correlation analysis suggested that the long non-coding RNA (lncRNA) AL049555.1 and the microRNA (miRNA) hsa-miR-324-5p were involved in the expression of RNF169, composing a potential regulatory axis to control the progression of PAAD. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that RNF169 plays a role in PAAD through pathways such as TNF, Hippo, JAK-STAT and Toll-like receptor signaling. Conclusion: In summary, the upregulation of RNF169 expression mediated by ncRNAs might influence immune cell infiltration in the microenvironment; thus, it can be used as a prognostic biomarker and a potential therapeutic target in PAAD.

引用

页数：16

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