Bulk RNA sequencing for analysis of post COVID-19 condition in adolescents and young adults

被引：1
作者：
Sommen, Silke Lauren [1 ,2 ]
Zhao, Zhi [3 ]
Segtnan, Sunniva [2 ]
Stiansen-Sonerud, Tonje [1 ,4 ,5 ]
Selvakumar, Joel [1 ,6 ]
Havdal, Lise Beier [1 ]
Gjerstad, Johannes [7 ]
Wyller, Vegard Bruun Bratholm [1 ,6 ]
Berven, Lise Lund [1 ]
机构：
[1] Akershus Univ Hosp, Dept Pediat, Lorenskog, Norway
[2] Univ Oslo, Oslo, Norway
[3] Univ Oslo, Inst Basic Med Sci, Fac Med, Oslo Ctr Biostat & Epidemiol OCBE, Oslo, Norway
[4] Univ Oslo, Dept Clin Mol Biol EpiGen, Lorenskog, Norway
[5] Akershus Univ Hosp, Lorenskog, Norway
[6] Univ Oslo, Inst Clin Med, Fac Med, Oslo, Norway
[7] Oslo Metropolitan Univ, Fac Hlth Sci, Dept Behav Sci, Oslo, Norway
来源：
JOURNAL OF TRANSLATIONAL MEDICINE | 2024年 / 22卷 / 01期
关键词：
Post COVID-19 condition; Long COVID; Child; Adolescent; RNA sequencing; Transcriptomics; Immunology; CHRONIC-FATIGUE-SYNDROME; GENE-EXPRESSION; INTERFERON;
D O I：
10.1186/s12967-024-05117-7
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background Post COVID-19 condition (PCC) is a complication of SARS-COV-2 infection and can lead to long-term disability.Methods The present study was designed to analyse the gene expression patterns of PCC through bulk RNA sequencing of whole blood and to explore the potential molecular mechanisms of PCC. Whole blood was collected from 80 participants enrolled in a prospective cohort study following SARS-CoV-2 infected and non-infected individuals for 6 months after recruitment and was used for bulk RNA sequencing. Identification of differentially expressed genes (DEG), pathway enrichment and immune cell deconvolution was performed to explore potential biological pathways involved in PCC.Results We have found 13 differentially expressed genes associated with PCC. Enriched pathways were related to interferon-signalling and anti-viral immune processes.Conclusion The PCC transcriptome is characterized by a modest overexpression of interferon-stimulated genes, pointing to a subtle ongoing inflammatory response.
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页数：13
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