Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis

被引：29
作者：
Zhang, Qiongqiong [1 ]
Deng, Peiying [1 ]
Chen, Suhui [1 ]
Xu, Hong [1 ]
Zhang, Yamin [1 ]
Chen, Hui [2 ,3 ,4 ,5 ,6 ]
Zhang, Jianmin [2 ,3 ,4 ,5 ,6 ,7 ]
Sun, Hua [1 ]
机构：
[1] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Peking Union Med Coll, Dept Tradit Chinese Med, Beijing, Peoples R China
[2] Chinese Acad Med Sci, Inst Basic Med Sci, Peking Union Med Coll, CAMS Key Lab T Cell & Immunotherapy,Dept Immunol,S, Beijing, Peoples R China
[3] Peking Union Med Coll, Sch Basic Med, Beijing, Peoples R China
[4] Chinese Acad Med Sci, Haihe Lab Cell Ecosyst, Tianjin, Peoples R China
[5] Peking Union Med Coll, Tianjin, Peoples R China
[6] Changzhou Xitaihu Inst Frontier Technol Cell Thera, Changzhou, Peoples R China
[7] Guidon Pharmaceut Inc, Beijing, Peoples R China
来源：
FRONTIERS IN IMMUNOLOGY | 2023年 / 14卷
基金：
中国国家自然科学基金;
关键词：
electroacupuncture; ischemic stroke; induced pluripotent stem cell; microbiome-gut-brain axis; inflammation; MOUSE MODEL; DYSBIOSIS; NEUROINFLAMMATION; ASSOCIATION; ACTIVATION; MANAGEMENT; COLITIS; UPDATE; CELLS;
D O I：
10.3389/fimmu.2023.1107559
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Electroacupuncture (EA) and induced pluripotent stem cell (iPSC)-derived small extracellular vesicles (iPSC-EVs) have substantial beneficial effects on ischemic stroke. However, the detailed mechanisms remain unclear. Here, we explored the mechanisms underlying the regulation of EA and iPSC-EVs in the microbiome-gut-brain axis (MGBA) after ischemic stroke. Ischemic stroke mice (C57BL/6) were subjected to middle cerebral artery occlusion (MCAO) or Sham surgery. EA and iPSC-EVs treatments significantly improved neurological function and neuronal and intestinal tract injury, downregulated the levels of IL-17 expression and upregulated IL-10 levels in brain and colon tissue after cerebral ischemia-reperfusion. EA and iPSC-EVs treatments also modulated the microbiota composition and diversity as well as the differential distribution of species in the intestines of the mice after cerebral ischemia-reperfusion. Our results demonstrated that EA and iPSC-EVs treatments regulated intestinal immunity through MGBA regulation of intestinal microbes, reducing brain and colon damage following cerebral ischemia and positively impacting the outcomes of ischemic stroke. Our findings provide new insights into the application of EA combined with iPSC-EVs as a treatment for ischemic stroke.
引用
收藏
页数：14
相关论文
共 74 条
[1]  
Abbafati C, 2020, LANCET, V396, P1204
[2]   Signaling inflammation across the gut-brain axis [J].
Agirman, Gulistan ;
Yu, Kristie B. ;
Hsiao, Elaine Y. .
SCIENCE, 2021, 374 (6571) :1087-1092
[3]   Brain-gut axis after stroke [J].
Arya, Awadhesh K. ;
Hu, Bingren .
BRAIN CIRCULATION, 2018, 4 (04) :165-173
[4]   Commensal microbiota affects ischemic stroke outcome by regulating intestinal γδ T cells [J].
Benakis, Corinne ;
Brea, David ;
Caballero, Silvia ;
Faraco, Giuseppe ;
Moore, Jamie ;
Murphy, Michelle ;
Sita, Giulia ;
Racchumi, Gianfranco ;
Lingo, Lilan ;
Pamer, Eric G. ;
Iadecola, Costantino ;
Anrather, Josef .
NATURE MEDICINE, 2016, 22 (05) :516-523
[5]   Akkermansia muciniphila: paradigm for next-generation beneficial microorganisms [J].
Cani, Patrice D. ;
Depommier, Clara ;
Derrien, Muriel ;
Everard, Amandine ;
de Vos, Willem M. .
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY, 2022, 19 (10) :625-637
[6]  
Chen Dan-Feng, 2022, Zhen Ci Yan Jiu, V47, P216, DOI 10.13702/j.1000-0607.20210766
[7]   Transplantation of fecal microbiota rich in short chain fatty acids and butyric acid treat cerebral ischemic stroke by regulating gut microbiota [J].
Chen, Runzhi ;
Xu, Ying ;
Wu, Peng ;
Zhou, Hao ;
Lasanajak, Yi ;
Fang, Yingying ;
Tang, Lan ;
Ye, Ling ;
Li, Xing ;
Cai, Zheng ;
Zhao, Jie .
PHARMACOLOGICAL RESEARCH, 2019, 148
[8]   Electroacupuncture promotes axonal regrowth by attenuating the myelin-associated inhibitors-induced RhoA/ROCK pathway in cerebral ischemia/reperfusion rats [J].
Chen, Suhui ;
Wang, Huanyuan ;
Xu, Hong ;
Zhang, Yamin ;
Sun, Hua .
BRAIN RESEARCH, 2020, 1748
[9]   Interactions Between the Gut Microbiota and the Host Innate Immune Response Against Pathogens [J].
Cheng, Hong-Yu ;
Ning, Meng-Xia ;
Chen, De-Kun ;
Ma, Wen-Tao .
FRONTIERS IN IMMUNOLOGY, 2019, 10
[10]   Electroacupuncture modulates the intestinal microecology to improve intestinal motility in spinal cord injury rats [J].
Cheng, Jie ;
Li, Weimin ;
Wang, Ying ;
Cao, Qing ;
Ni, Ying ;
Zhang, Wenyi ;
Guo, Jiabao ;
Chen, Binglin ;
Zang, Yaning ;
Zhu, Yi .
MICROBIAL BIOTECHNOLOGY, 2022, 15 (03) :862-873
12345678