Genome evolution of dengue virus serotype 1 under selection by Wolbachia pipientis in Aedes aegypti mosquitoes

被引:10
作者
Kien, Duong Thi Hue [1 ]
Edenborough, Kathryn [2 ,3 ]
Goncalves, Daniela da Silva [2 ]
Vi, Tran Thuy [1 ]
Casagrande, Etiene [2 ,4 ]
Duyen, Huynh Thi Le [1 ]
Long, Vo Thi [1 ]
Dui, Le Thi [1 ]
Nhu, Vu Thi Tuyet [1 ]
Giang, Nguyen Thi [1 ]
Trang, Huynh Thi Xuan [1 ]
Lee, Elvina [2 ]
Donovan-Banfield, I'ah [2 ]
Van, Huynh Thi Thuy [1 ]
Nguyet, Nguyen Minh [1 ]
Phong, Nguyen Thanh [1 ,5 ]
Chau, Nguyen Van Vinh [1 ]
Wills, Bridget [1 ,6 ]
Yacoub, Sophie [1 ]
Flores, Heather [2 ,4 ]
Simmons, Cameron [2 ,3 ]
机构
[1] Univ Oxford, Hosp Trop Dis, Clin Res Unit, Ho Chi Minh City, Vietnam
[2] Monash Univ, Inst Vector Borne Dis, World Mosquito Program, Clayton, Vic 3800, Australia
[3] Monash Univ, Biomed Discovery Inst, Dept Microbiol, Clayton, Vic 3800, Australia
[4] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia
[5] Hosp Trop Dis, 190 Ben Ham Tu, Ho Chi Minh City, Vietnam
[6] Univ Oxford, Nuffield Dept Med, Oxford, England
关键词
dengue virus; wMel Wolbachia; virus evolution; Aedes aegypti mosquitoes; biocontrol; INFECTION; BINDING;
D O I
10.1093/ve/vead016
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The introgression of antiviral strains of Wolbachia into Aedes aegypti mosquito populations is a public health intervention for the control of dengue. Plausibly, dengue virus (DENV) could evolve to bypass the antiviral effects of Wolbachia and undermine this approach. Here, we established a serial-passage system to investigate the evolution of DENV in Ae. aegypti mosquitoes infected with the wMel strain of Wolbachia. Using this system, we report on virus genetic outcomes after twenty passages of serotype 1 of DENV (DENV-1). An amino acid substitution, E203K, in the DENV-1 envelope protein was more frequently detected in the consensus sequence of virus populations passaged in wMel-infected Ae. aegypti than wild-type counterparts. Positive selection at residue 203 was reproducible; it occurred in passaged virus populations from independent DENV-1-infected patients and also in a second, independent experimental system. In wild-type mosquitoes and human cells, the 203K variant was rapidly replaced by the progenitor sequence. These findings provide proof of concept that wMel-associated selection of virus populations can occur in experimental conditions. Field-based studies are needed to explore whether wMel imparts selective pressure on DENV evolution in locations where wMel is established.
引用
收藏
页数:10
相关论文
共 52 条
[1]   Wolbachia enhances insect-specific flavivirus infection in Aedes aegypti mosquitoes [J].
Amuzu, Hilaria E. ;
Tsyganov, Kirill ;
Koh, Cassandra ;
Herbert, Rosemarie I. ;
Powell, David R. ;
McGraw, Elizabeth A. .
ECOLOGY AND EVOLUTION, 2018, 8 (11) :5441-5454
[2]   Field-and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes [J].
Carrington, Lauren B. ;
Bich Chau Nguyen Tran ;
Nhat Thanh Hoang Le ;
Tai Thi Hue Luong ;
Truong Thanh Nguyen ;
Phong Thanh Nguyen ;
Chau Van Vinh Nguyen ;
Huong Thi Cam Nguyen ;
Trung Tuan Vu ;
Long Thi Vo ;
Dui Thi Le ;
Nhu Tuyet Vu ;
Giang Thi Nguyen ;
Hung Quoc Luu ;
Anh Duc Dang ;
Hurst, Timothy P. ;
O'Neill, Scott L. ;
Vi Thuy Tran ;
Duong Thi Hue Kien ;
Nguyet Minh Nguyen ;
Wolbers, Marcel ;
Wills, Bridget ;
Simmons, Cameron P. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (02) :361-366
[3]  
Chaudhury Sidhartha, 2015, Biochem Biophys Rep, V4, P375, DOI 10.1016/j.bbrep.2015.10.014
[4]   ALIENTRIMMER: A tool to quickly and accurately trim off multiple short contaminant sequences from high-throughput sequencing reads [J].
Criscuolo, Alexis ;
Brisse, Sylvain .
GENOMICS, 2013, 102 (5-6) :500-506
[5]   wMel Wolbachia genome remains stable after 7 years in Australian Aedes aegypti field populations [J].
Dainty, Kimberley R. ;
Hawkey, Jane ;
Judd, Louise M. ;
Pacidonio, Etiene C. ;
Duyvestyn, Johanna M. ;
Goncalves, Daniela S. ;
Lin, Silk Yu ;
O'Donnell, Tanya B. ;
O'Neill, Scott L. ;
Simmons, Cameron P. ;
Holt, Kathryn E. ;
Flores, Heather A. .
MICROBIAL GENOMICS, 2021, 7 (09)
[6]   Using Wolbachia to Eliminate Dengue: Will the Virus Fight Back? [J].
Edenborough, Kathryn M. ;
Flores, Heather A. ;
Simmons, Cameron P. ;
Fraser, Johanna E. .
JOURNAL OF VIROLOGY, 2021, 95 (13)
[7]   Modeling the impact on virus transmission of Wolbachia-mediated blocking of dengue virus infection of Aedes aegypti [J].
Ferguson, Neil M. ;
Duong Thi Hue Kien ;
Clapham, Hannah ;
Aguas, Ricardo ;
Vu Tuan Trung ;
Tran Nguyen Bich Chau ;
Popovici, Jean ;
Ryan, Peter A. ;
O'Neill, Scott L. ;
McGraw, Elizabeth A. ;
Vo Thi Long ;
Le Thi Dui ;
Nguyen, Hoa L. ;
Nguyen Van Vinh Chau ;
Wills, Bridget ;
Simmons, Cameron P. .
SCIENCE TRANSLATIONAL MEDICINE, 2015, 7 (279)
[8]   Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti [J].
Flores, Heather A. ;
Taneja de Bruyne, Jyotika ;
O'Donnell, Tanya B. ;
Tuyet Nhu, Vu ;
Thi Giang, Nguyen ;
Thi Xuan Trang, Huynh ;
Thi Thuy Van, Huynh ;
Thi Long, Vo ;
Thi Dui, Le ;
Le Anh Huy, Huynh ;
Thi Le Duyen, Huynh ;
Thi Van Thuy, Nguyen ;
Thanh Phong, Nguyen ;
Van Vinh Chau, Nguyen ;
Thi Hue Kien, Duong ;
Thuy Vi, Tran ;
Wills, Bridget ;
O'Neill, Scott L. ;
Simmons, Cameron P. ;
Carrington, Lauren B. .
PLOS PATHOGENS, 2020, 16 (04)
[9]   Vector-Borne Transmission Imposes a Severe Bottleneck on an RNA Virus Population [J].
Forrester, Naomi L. ;
Guerbois, Mathilde ;
Seymour, Robert L. ;
Spratt, Heidi ;
Weaver, Scott C. .
PLOS PATHOGENS, 2012, 8 (09)
[10]   Novel phenotype of Wolbachia strain wPip in Aedes aegypti challenges assumptions on mechanisms of Wolbachia-mediated dengue virus inhibition [J].
Fraser, Johanna E. ;
O'Donnell, Tanya B. ;
Duyvestyn, Johanna M. ;
O'Neill, Scott L. ;
Simmons, Cameron P. ;
Flores, Heather A. .
PLOS PATHOGENS, 2020, 16 (07)