Genome Investigation and Functional Annotation of Lactiplantibacillus plantarum YW11 Revealing Streptin and Ruminococcin-A as Potent Nutritive Bacteriocins against Gut Symbiotic Pathogens

被引：33

作者：

Aziz, Tariq ^{[1
,2
]}

Naveed, Muhammad ^{[3
]}

Makhdoom, Syeda Izma ^{[3
]}

Ali, Urooj ^{[3
]}

Mughal, Muhammad Saad ^{[3
]}

Sarwar, Abid ^{[1
]}

Khan, Ayaz Ali ^{[4
]}

Zhennai, Yang ^{[1
]}

Sameeh, Manal Y. ^{[5
]}

Dablool, Anas S. ^{[6
]}

Alharbi, Amnah A. ^{[7
]}

Shahzad, Muhammad ^{[2
]}

Alamri, Abdulhakeem S. ^{[8
,9
]}

Alhomrani, Majid ^{[8
,9
]}

机构：

[1] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing Engn & Technol Res Ctr Food Addit, Beijing 100048, Peoples R China

[2] Khyber Med Univ, Inst Basic Med Sci, Peshawar 25100, Pakistan

[3] Univ Cent Punjab, Fac Sci & Technol, Dept Biotechnol, Lahore 54590, Pakistan

[4] Univ Malakand, Dept Biotechnol, Chakdara 18800, Pakistan

[5] Umm Al Qura Univ, Al Leith Univ Coll, Fac Appl Sci, Chem Dept, Mecca 24831, Saudi Arabia

[6] Umm Al Qura Univ, Hlth Sci Coll Al Leith, Dept Publ Hlth, Mecca 24382, Saudi Arabia

[7] Univ Tabuk, Fac Sci, Dept Biochem, Tabuk 71491, Saudi Arabia

[8] Taif Univ, Fac Appl Med Sci, Dept Clin Lab Sci, Taif 21944, Saudi Arabia

[9] Taif Univ, Ctr Biomed Sci Res CBSR, Deanship Sci Res, Taif 21944, Saudi Arabia

来源：

MOLECULES | 2023年 / 28卷 / 02期

基金：

中国国家自然科学基金;

关键词：

LP YW11; whole genome; streptin; ruminococcin; nutritive bacteriocin; gut microbiota; IN-SILICO CHARACTERIZATION; LACTOBACILLUS-PLANTARUM; PROBIOTIC BACTERIA; LINOLEIC-ACID; EXOPOLYSACCHARIDES;

D O I：

10.3390/molecules28020491

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

All nutrient-rich feed and food environments, as well as animal and human mucosae, include lactic acid bacteria known as Lactobacillus plantarum. This study reveals an advanced analysis to study the interaction of probiotics with the gastrointestinal environment, irritable bowel disease, and immune responses along with the analysis of the secondary metabolites' characteristics of Lp YW11. Whole genome sequencing of Lp YW11 revealed 2297 genes and 1078 functional categories of which 223 relate to carbohydrate metabolism, 21 against stress response, and the remaining 834 are involved in different cellular and metabolic pathways. Moreover, it was found that Lp YW11 consists of carbohydrate-active enzymes, which mainly contribute to 37 glycoside hydrolase and 28 glycosyltransferase enzyme coding genes. The probiotics obtained from the BACTIBASE database (streptin and Ruminococcin-A bacteriocins) were docked with virulent proteins (cdt, spvB, stxB, and ymt) of Salmonella, Shigella, Campylobacter, and Yersinia, respectively. These bacteria are the main pathogenic gut microbes that play a key role in causing various gastrointestinal diseases. The molecular docking, dynamics, and immune simulation analysis in this study predicted streptin and Ruminococcin-A as potent nutritive bacteriocins against gut symbiotic pathogens.

引用

页数：19

共 35 条

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