Investigation of vancomycin resistance genes in Enterococcus species isolated from bovine mastitis

被引:0
作者
Seker, Esra [1 ,4 ]
Ozenc, Erhan [2 ]
Yilmaz, Muesser [3 ]
机构
[1] Afyon Kocatepe Univ, Fac Vet Med, Dept Microbiol, Afyonkarahisar, Turkiye
[2] Afyon Kocatepe Univ, Fac Vet Med, Dept Obstet & Gynecol, Afyonkarahisar, Turkiye
[3] Republ Turkey Minist Agr & Forestry, Karacoban Dist Directorate Agr & Forestry, Erzurum, Turkiye
[4] Afyon Kocatepe Univ, Fac Vet Med, Dept Microbiol, ANS Campus, TR-03200 Afyonkarahisar, Turkiye
关键词
antimicrobial resistance; bovine mastitis; Enterococcus spp; PCR; vancomycin resistance; ANTIBIOTIC-RESISTANCE; GLYCOPEPTIDE RESISTANCE; ANTIMICROBIAL RESISTANCE; MULTIPLEX PCR; FOOD ANIMALS; HUMAN HEALTH; VIRULENCE; PREVALENCE; SAMPLES; MILK;
D O I
10.24099/vet.arhiv.1697
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The aim of the present study was to isolate Enterococcus species from mastitis in cows, investigate the vancomycin resistance genes in the isolated species using PCR, and determine the antibiotic resistance of VRE strains to some antibiotics commonly used in Turkey. A total of 512 mammary quarter milk samples from 150 lactating cows were used. Following phenotypic typing by a commercial identification kit, multiplex PCR was applied to the strains using species specific primers. The Enterococcus isolation rate was found to be 13.9% (n=71). The most frequently isolated species was E. faecalis (n=40, 56.3%), followed by E. faecium (n=16, 22.5%), E. solitarius (n=6, 8.5%), E. durans (n=5, 7.1%) and E. hirae (n=4, 5.6%). Of 71 Enterococcus strains, 19 (26.7%) were determined to be VRE. While a total of five vanA (7%), 10 vanB (14%) and 12 vanC2/C3 (16.9%) genes were detected in the strains, none of the strains harbored the vanC1 gene. The vancomycin resistance gene was not found in any E. solitarius strain. While all of the VR 19 strains were phenotypically resistant to vancomycin and fusidic acid, high resistance rates were also determined in the strains to streptomycin (84.2%), erythromycin (73.7%), tetracycline (68.4%), penicillin G (68.4%), gentamicin (68.4%), lincomycin (57.9%), cephalothin (52.6%) and kanamycin (52.6%). Consequently, it was thought the VRE positive mastitic milk samples may comprise a potential risk for public health. To our knowledge, this is the first study showing the presence of VRE in milk with mastitis by PCR in Afyonkarahisar.
引用
收藏
页码:287 / 298
页数:12
相关论文
共 44 条
[1]   Molecular epidemiology of hospital-acquired vancomycin-resistant enterococci [J].
Abele-Horn, Marianne ;
Vogel, Ulrich ;
Klare, Ingo ;
Konstabel, Carola ;
Trabold, Rene ;
Kurihara, Risa ;
Witte, Wolfgang ;
Kreth, Wolfgang ;
Schlegel, Paul-Gerhardt ;
Claus, Heike .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (11) :4009-4013
[2]  
Adams Daniel J, 2016, Hosp Pediatr, V6, P404, DOI 10.1542/hpeds.2015-0196
[3]  
Attia A. M., 2017, Zagazig Veterinary Journal, V45, P64
[4]   Bovine mastitis: a persistent and evolving problem requiring novel approaches for its control - a review [J].
Benic, Miroslav ;
Macesic, Nino ;
Cvetnic, Luka ;
Habrun, Boris ;
Cvetnic, Zeljko ;
Turk, Romana ;
Duricic, Drazen ;
Lojkic, Martina ;
Dobranic, Vesna ;
Valpotic, Hrvoje ;
Grizelj, Juraj ;
Gracner, Damjan ;
Grbavac, Jozo ;
Samardzija, Marko .
VETERINARSKI ARHIV, 2018, 88 (04) :535-557
[5]   Emergence of vancomycin-resistant enterococci in Switzerland: a nation-wide survey [J].
Buetti, Niccolo ;
Wassilew, Nasstasja ;
Rion, Viktorija ;
Senn, Laurence ;
Gardiol, Celine ;
Widmer, Andreas ;
Marschall, Jonas ;
Balmelli, Carlo ;
Eisenring, Marie-Christine ;
Harbarth, Stephan ;
Kuster, Stefan P. ;
Marschall, Jonas ;
Spicher, Virginie Masserey ;
Pittet, Didier ;
Ruef, Christian ;
Sax, Hugo ;
Schlegel, Matthias ;
Schweiger, Alexander ;
Troillet, Nicolas ;
Widmer, Andreas F. ;
Zanetti, Giorgio .
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL, 2019, 8 (1)
[6]  
Burovic Jasmin, 2020, Veterinarska Stanica, V51, P47
[7]  
Centers for Disease Control and Prevention (CDC), 1993, MMWR Morb Mortal Wkly Rep, V42, P597
[8]   MOVABLE GENETIC ELEMENTS AND ANTIBIOTIC-RESISTANCE IN ENTEROCOCCI [J].
CLEWELL, DB .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1990, 9 (02) :90-102
[9]  
Clinical and Laboratory Standards Institute ( CLSI), 2017, M100 CLSI
[10]  
Clinical and Laboratory Standards Institute (CLSI), 2013, CLSI M100-S23