Molecular typing of Staphylococcus aureus isolated from bovine and poultry based on PCR-RFLP of spa gene

Staphylococcus aureus (S. aureus) is considered one of the most important pathogen in bovine and poultry. The aim of the present study was to assess the genotypic polymorphism among S. aureus isolate were recov-ered from bovine and poultry in a geographically restricted area by PCR-RFLP of the spa gene. Fifty-eight isolates of S. aureus were obtained from bovine mastitis (n=30) and poultry (n=28) during six months. Identification of the isolates was done using conventional methods then confirmed by species-specific PCR method. The spa typing was performed by amplification of the spa gene X region. Digestion of the amplified spa gene fragment with Bsp143I en-donuclease produced 4 to 5 bands. Dendrogram obtained by RAPD results revealed eight RAPD clusters (named as A to H).Isolates with more than 70% similarity were clustered together as a same cluster. Cluster A contains most S. aureus isolates of bovine origin and cluster B contains most isolates of poultry origin. Only F and H cluster contained both poultry and bovine isolates (7/58, 12%).Our results confirmed that RFLP of spa gene product using Bsp143I enzyme can be applied perfectly to differentiate bovine's and chicken's S. aureus isolates, also supporting the idea that S. aureus isolates with different genotype may constitute a distinct infection.