Enzymatic synthesis of mono- and disubstituted phospholipids by direct condensation of oleic acid and glycerophosphocholine with immobilized lipases and phospholipase

Lysophospholipids which contain polyunsaturated fatty acids play a key role in food and cosmetic industries because of their bioactivity. Therefore, the formation of mono-and disubstituted phospholipids is quite inter-esting as they could be used for the formation of different natural liposomes.Using immobilized derivatives of lipases and phospholipases, the esterification of oleic acid with glycer-ophosphocholine (GPC) has been studied. Thus, derivatives were quite active in completely anhydrous media and in solvent-free reaction systems where the reaction takes place.CALB biocatalyst was able to successfully form oleoyl-LPC at 60 degrees C in the presence of 30 % butanone, where the synthesis rate was 100 times higher than in the absence of solvents at 40 degrees C. On the other hand, the best synthesis rate for dioleoyl-PC was achieved with immobilized Lecitase in a solvent-free process at 60 degrees C, an 83 % synthesis yield was achieved with an initial synthesis rate of 4.32 mg/mL x h x g.