Functional characterization of 2-oxoglutarate-dependent dioxygenase gene family in chickpea

被引:5
作者
Saxena, Samiksha [1 ]
Pal, Gaurav [2 ]
Pandey, Ashutosh [1 ]
机构
[1] Natl Inst Plant Genome Res, Aruna Asaf Ali Marg, New Delhi 110067, India
[2] North Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC USA
关键词
Chickpea; Biosynthesis pathway; Gene complementation; Bifunctionality; Metabolite content; CICER-ARIETINUM L; FLAVONOL SYNTHASE; BIOSYNTHESIS; EXPRESSION; PATHWAY; IDENTIFICATION; EVOLUTION; ENZYMES; LEADS;
D O I
10.1016/j.plantsci.2023.111836
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chickpea is an important leguminous crop plant with two cultivated types, desi and kabuli. It is nutritionally enriched in flavonoid content in addition to minerals and vitamins imparting huge health benefits to human beings. Our study elucidates the functionality of 2-oxoglutarate dependent dioxygenase (2-ODD) gene family members i.e., flavanone-3-hydroxylase (F3H), flavonol synthase (FLS) and anthocyanidin synthase (ANS) in chickpea using heterologous bacterial system and in-planta studies in Arabidopsis. This provides information about the biosynthesis of two very significant sub-classes of flavonoids- flavonols and anthocyanins. Here, we show that all the three homologs of F3H in chickpea can utilize not just naringenin but also eriodictyol as their substrate. Moreover, we show that FLS in chickpea exhibits bifunctionality having both FLS and F3H activity. Also, our study indicates the richness of desi chickpea over kabuli type through gene expression and metabolite content analyses. Overall, our study establishes the functionality of 2-ODD gene family involved in the early and late steps of flavonoid biosynthesis pathway in chickpea. It paves way for better genetic manipulation of the pathway for direct or indirect synthesis of three major subclasses of flavonoids (flavonol, anthocyanin and proanthocyanin) to develop nutritious, environmentally stable and healthy chickpea (Cicer arietinum) crop.
引用
收藏
页数:12
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